Abstract | BACKGROUND AND PURPOSE: METHODS: RESULTS:
IGF-1 gene transfer compared with control treatment significantly improved motor performance assessed by sensorimotor tests. The functional recovery was accompanied by reduced volume of cerebral infarction. Immunohistochemical analysis with endothelial cell marker CD31 revealed that IGF-1 gene transfer potently increased neovessel formation in the periinfarct and injection needle tract area compared with AAV- green fluorescent protein transduction. Increased vascular density was associated with increased local vascular perfusion. Additionally, AAV-IGF-1 treatment enhanced neurogenesis in the subventricular zone compared with AAV- green fluorescent protein treatment. CONCLUSIONS: These data demonstrate that IGF-1 overexpression promoted long-lasting functional recovery after cerebral infarction. The improved functional performance was paralleled by enhanced neovascularization and neurogenesis.
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Authors | Wei Zhu, Yongfeng Fan, Tim Frenzel, Mehdi Gasmi, Raymond T Bartus, William L Young, Guo-Yuan Yang, Yongmei Chen |
Journal | Stroke
(Stroke)
Vol. 39
Issue 4
Pg. 1254-61
(Apr 2008)
ISSN: 1524-4628 [Electronic] United States |
PMID | 18309153
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Insulin-Like Growth Factor I
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Topics |
- Animals
- Atrophy
- Cell Division
(physiology)
- Cerebrovascular Circulation
(physiology)
- Dependovirus
(genetics)
- Disease Models, Animal
- Genetic Therapy
(methods)
- Infarction, Middle Cerebral Artery
(pathology, therapy)
- Insulin-Like Growth Factor I
(genetics)
- Mice
- Mice, Inbred Strains
- Neovascularization, Physiologic
(physiology)
- Neurons
(cytology)
- Recovery of Function
(physiology)
- Transduction, Genetic
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