Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism.

Abstract

A method to determine the catecholamine content in putamen (CPU) and midbrain (MB) regions of the brain of alcohol-preferring rats (P) is presented with a focus on the low-level detection of S,R-salsolinol, a metabolite of dopamine and a putative alcoholism marker. The developed strategy allows both quantitative profiling of related catecholamines and the enantiomeric separation and quantification of the S- and R-salsolinol isomers and their ratios. The described LC/MS strategy simplifies the current methodology that typically employs GC-MS by eliminating the need for derivatization. The data also suggest an increase in the non-enzymatic formation of salsolinol as a consequence of ethanol exposure.

• Authors: Tatiana Rojkovicova, Yehia Mechref, Jason A Starkey, Guangxiang Wu, Richard L Bell, William J McBride, Milos V Novotny
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 863 Issue 2 Pg. 206-14 (Mar 01 2008) ISSN: 1570-0232 [Print] Netherlands
• PMID: 18272438 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Validation Study)

Chemical References

• Cyclodextrins
• Isoquinolines
• cyclodextrin polymer
• Cellulose
• salsolinol
• Dopamine

Topics

• Alcoholism (metabolism)
• Animals
• Brain Chemistry
• Cellulose (chemistry)
• Chromatography, Liquid (methods)
• Cyclodextrins (chemistry)
• Dopamine (metabolism)
• Genetic Predisposition to Disease
• Isoquinolines (analysis, chemistry)
• Male
• Mass Spectrometry (methods)
• Mesencephalon (chemistry, metabolism)
• Molecular Structure
• Putamen (chemistry, metabolism)
• Rats
• Rats, Inbred Strains
• Reproducibility of Results
• Sensitivity and Specificity
• Stereoisomerism