Curcumin attenuates ischemia-reperfusion injury in rat testis.

Abstract

OBJECTIVE:

To evaluate the effect of curcumin, a potent antioxidant, on testicular ischemia-reperfusion injury caused by overgeneration of reactive oxygen species (ROS) after testicular torsion-detorsion.

DESIGN:

Controlled experimental study using rats.

SETTING:

Research laboratory.

ANIMAL(S):

Sixty adult male Sprague-Dawley rats.

INTERVENTION(S):

Rats in the control group underwent a sham operation of the left testis. In the torsion-detorsion group, the left testis was rotated 720 degrees for 2 hours. Rats in treatment group received the same surgical procedure as the torsion-detorsion group, but curcumin was administered IV at repair of testicular torsion.

MAIN OUTCOME MEASURE(S):

Testicular activity of xanthine oxidase, which catalyzes production of ROS; malondialdehyde level (an indicator of ROS content); protein expression level of heme oxygenase-1, which catalyzes antioxidant generation; and spermatogenesis.

RESULT(S):

Unilateral testicular torsion-detorsion caused significant increases in xanthine oxidase activity, malondialdehyde level, and heme oxygenase-1 protein expression level and caused a significant decrease in testicular spermatogenesis in ipsilateral testes. The rats treated with curcumin had significant decreases in xanthine oxidase activity and malondialdehyde level and had significant increases in heme oxygenase-1 protein expression level and testicular spermatogenesis in ipsilateral testes, compared with the torsion-detorsion group.

CONCLUSION(S):

The curcumin exerts a protective effect on testicular ischemia-reperfusion injury.

• Authors: Si-Ming Wei, Zhi-Zhong Yan, Jian Zhou
• Journal: Fertility and sterility (Fertil Steril) Vol. 91 Issue 1 Pg. 271-7 (Jan 2009) ISSN: 1556-5653 [Electronic] United States
• PMID: 18222428 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Reactive Oxygen Species
• Malondialdehyde
• Xanthine Oxidase
• Curcumin

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (therapeutic use)
• Curcumin (therapeutic use)
• Disease Models, Animal
• Male
• Malondialdehyde (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)
• Reperfusion Injury (enzymology, prevention & control)
• Spermatic Cord Torsion (prevention & control)
• Testis (blood supply, drug effects, physiopathology)
• Xanthine Oxidase (metabolism)