Abstract |
Precursors of B cells capable of responding to a T-independent form of phosphorylcholine (PC) in splenic focus assays were detected in the spleens of neonatal mice as early as 4 days after birth. The earliest anti-PC B cells were T15-. T15+ foci-forming B cells were first detected 6 days after birth and expanded rapidly to constitute greater than 80% of the total PC-specific foci by day 10. Injection of heat-killed S. pneumoniae (R36A) into neonatal mice resulted in priming of the antibody response to PC, with an idiotype profile reflecting that of precursors of foci-forming B cells at the time of antigen administration. Priming of 2-day-old mice with 2 x 10(6) and 2 x 10(7) R36A induced a five- and ten-fold increase in the antibody response to phosphorylcholine 6 to 8 weeks later. However, only 10 to 15% of the serum antibodies expressed the normally dominant T15 idiotype. Doses below 2 x 10(5) R36A showed no detectable priming activity. PC-specific hybridomas derived from mice injected with 2 x 10(7) R36A 2 days after birth lacked the idiotypic and molecular characteristics typical of T15+ antibodies. Antibodies to phosphorylcholine, raised by immunization of 6-week-old mice are normally protective against pneumococcal infection. However, serum antibodies from mice treated with R36A 2 days after birth and responding to phosphorylcholine following challenge with R36A at 6 weeks of age failed to protect against deliberate infection with virulent S. pneumoniae. These observations imply that the antigen phosphorylcholine does not play a role in the selective expansion and dominant expression of the T15 idiotype.
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Authors | M Vakil, D E Briles, J F Kearney |
Journal | Developmental immunology
(Dev Immunol)
Vol. 1
Issue 3
Pg. 203-12
( 1991)
ISSN: 1044-6672 [Print] England |
PMID | 1821697
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Bacterial
- Antigens, Bacterial
- Antigens, T-Independent
- Immunoglobulin Idiotypes
- Phosphorylcholine
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Topics |
- Animals
- Animals, Newborn
(growth & development, immunology)
- Animals, Suckling
(growth & development, immunology)
- Antibodies, Bacterial
(biosynthesis, classification, immunology)
- Antigens, Bacterial
(immunology)
- Antigens, T-Independent
(immunology)
- B-Lymphocytes
(cytology, immunology)
- Cell Differentiation
- Clone Cells
(immunology)
- Immunization
- Immunoglobulin Idiotypes
(immunology)
- Mice
(growth & development, immunology)
- Mice, Inbred BALB C
(growth & development, immunology)
- Mice, Inbred CBA
(growth & development, immunology)
- Mice, Inbred DBA
(growth & development, immunology)
- Phosphorylcholine
(immunology)
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