Abstract | OBJECTIVE: STUDY DESIGN: Twenty-four women with CIN 1-3 received 4 mmol/L or 10 mmol/L HAL dissolved in thermolabile pluronic F 127 gel topically 5-720 minutes before surgical conization. The microscopic fluorescence pattern was quantified by ex vivo fluorescence spectroscopy at a wavelength of 635 nm and semiquantitatively by digital image analysis from cryosections. RESULTS: With 4 and 10 mmol/L HAL, porphyrin fluorescence intensity increased over time, reaching a peak after 180-540 minutes application intervals in CIN and normal epithelium. However, the peak was much more pronounced in CIN than in normal tissues. The fluorescence intensity with 10 mmol/L was significantly higher than that with 4 mmol/L in CIN tissues (P < .05). Maximum intensity could generally be detected in the lower layer of the epithelium. CONCLUSION: HAL seems to be a promising molecule for fluorescence diagnosis. For further treatment studies, we recommend application of 10 mmol/L HAL 300-540 minutes before photodynamic therapy in CIN.
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Authors | Peter Hillemanns, Xiuli Wang, Hermann Hertel, Vaagn Andikyan, Marija Hillemanns, Herbert Stepp, Philipp Soergel |
Journal | American journal of obstetrics and gynecology
(Am J Obstet Gynecol)
Vol. 198
Issue 3
Pg. 300.e1-7
(Mar 2008)
ISSN: 1097-6868 [Electronic] United States |
PMID | 18177838
(Publication Type: Journal Article)
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Chemical References |
- Carbon Radioisotopes
- Porphyrins
- Aminolevulinic Acid
- 5-aminolevulinic acid hexyl ester
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Topics |
- Administration, Topical
- Adult
- Aminolevulinic Acid
(administration & dosage, analogs & derivatives, pharmacokinetics)
- Carbon Radioisotopes
(administration & dosage, pharmacokinetics)
- Female
- Fluorescence
- Humans
- Middle Aged
- Photochemotherapy
- Porphyrins
(analysis, biosynthesis)
- Uterine Cervical Neoplasms
(diagnosis)
- Uterine Cervical Dysplasia
(diagnosis)
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