In order to develop a better
wound-dressing to enhance diabetic wound healing, we have examined the biochemical and biophysical features of
chitosan-crosslinked
collagen sponge (CCCS) and pre-clinically evaluated the CCCS containing recombinant human
acidic fibroblast growth factor (CCCS/FGF) in accelerating diabetic wound healing as compared to
collagen sponge alone and FGF alone.
Collagen crosslinked with
chitosan showed several advantages required for
wound dressing, including the uniform and porous ultrastructure, less water-imbibition, small interval porosity, high resistance to
collagenase digestion and slow release of FGF from CCCS/FGF.
Therapeutic effect of the new
wound-dressing containing FGF (i.e.: CCCS/FGF) on diabetic wound healing was examined in type 1 diabetic rat model in which
hyperglycemia was induced by single dose of
streptozotocin (STZ) and persisted for two months. The CCCS/FGF provided the most efficiently
therapeutic effect among various treatments, showing the shortest healing time (14 days in the CCCS/FGF-treated group as compared to 18~21 days in other treatment groups), the quickest tissue
collagen generation, the earliest and highest
TGF-beta1 expression and dermal cell proliferation (
PCNA expression). All these results suggest that CCCS/FGF is an ideal
wound-dressing to improve the recovery of healing-impaired
wound such as diabetic skin
wound, which provides a great potential use in clinics for diabetic patients in the future.