Abstract | BACKGROUND: OBJECTIVE: METHODS: BALB/c mice were sensitized by gavages with glycinin and beta-conglycinin, and allergen-specific IgE and IgG1 responses were studied by a passive cutaneous anaphylaxis assay. Serum histamine release and blood pressure were measured according to other methods. Epithelium and mast cell dye used the method of light microscopy. RESULTS: Sensitization with soybean allergens induced high levels of antigen-specific IgE and IgG1 and increased serum histamine in BALB/c mice. Percentiles of intact mast cell of small intestine in mice sensitized with glycinin and beta-conglyinin significantly decreased for 28 days. Degranulation of mast cells and damage of the epithelium in the small intestine of mice sensitized with globulins were observed. The level of blood pressure in sensitized mice reached a minimum at 3 h. CONCLUSION: Soybean-specific IgE and IgG1 antibodies increased, with high levels of histamine release, severe degranulation of mast cells and damage of the epithelium of small intestine in mice sensitized with glycinin and beta-conglyinin.
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Authors | X Liu, J Feng, Z R Xu, Y Z Wang, J X Liu |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 38
Issue 2
Pg. 350-6
(Feb 2008)
ISSN: 1365-2222 [Electronic] England |
PMID | 18070164
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Plant
- Globulins
- Immunoglobulin G
- Seed Storage Proteins
- Soybean Proteins
- beta-conglycinin protein, Glycine max
- Immunoglobulin E
- glycinin
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Topics |
- Anaphylaxis
(immunology)
- Animals
- Antigens, Plant
(immunology)
- Food Hypersensitivity
(immunology)
- Globulins
(administration & dosage, immunology)
- Immunoglobulin E
(blood)
- Immunoglobulin G
(blood)
- Intestines
(immunology)
- Mast Cells
(immunology)
- Mice
- Mice, Inbred BALB C
- Seed Storage Proteins
- Soybean Proteins
(administration & dosage, immunology)
- Syndrome
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