[Detection of mite allergens in the dust of filter-net and air of air-conditioned room].

Abstract

OBJECTIVE:

To detect mite allergens in the dust of air conditioner filter-net and floating air in room.

METHODS:

Samples were collected from rooms of asthma patient and normal families with or without air conditioner. Der p1, Der f1 and Der 2 were determined by two monoclonal antibody-based ELISA.

RESULTS:

In asthma patient families, the concentration of airborne Der p1, Der f1 and Der 2 was (0.23 +/- 0.13), (2.62 +/- 1.08), (0.93 +/- 0.41) ng/m3, and (0.56 +/- 0.25), (4.74 +/- 1.22), (2.33 +/- 0.64) ng/m3 respectively before and after the air conditioner switched on, all showing a significant difference (P < 0.05). In families without asthma patient, the concentration of Der p1, Der f1 and Der 2 was (0.33 +/- 0.11), (11.50 +/- 3.08) and (2.10 +/- 0.80) ng/m3, and (0.63 +/- 0.23), (19.80 +/- 4.30) and (3.60 +/- 1.00) ng/m3 respectively before and after the air conditioner switched on, also showing a significant difference (P < 0.05). From the filter-net dust of air conditioner in family with allergic asthma patient, the concentration of Der p1, Der f1 and Der 2 was (0.52 +/- 0.19), (3.34 +/- 0.63), (2.53 +/- 0.65) microg/g dust, while that of normal families was (1.30 +/- 0.35), (5.16 +/- 0.92), (3.47 +/- 1.13) microg/g dust respectively. The concentration of Der f1 and Der 2 at both asthma families and normal families was higher than 2 microg/g, an allergen concentration threshold.

CONCLUSION:

Mite allergens exist in the filter-net dust of air conditioner, which may be an important source of indoor allergens and a cause of the increasing prevalence of allergic asthma.

• Authors: Yu-yin Lian, Zhi-gang Liu, Hong-yu Wang, Cheng-yu Chai, Xiao-yu Liu
• Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi) Vol. 25 Issue 4 Pg. 325-7, 332 (Aug 2007) ISSN: 1000-7423 [Print] China
• PMID: 18038805 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Air Pollutants
• Allergens
• Dust

Topics

• Air Conditioning
• Air Pollutants (analysis, isolation & purification)
• Air Pollution, Indoor (analysis)
• Allergens (analysis, isolation & purification)
• Animals
• Asthma (immunology)
• Bedding and Linens
• Dust (analysis)
• Enzyme-Linked Immunosorbent Assay
• Filtration (instrumentation)
• Housing
• Humans
• Mites (immunology)