Abstract |
Laser confocal microscopy showed that fluorescence of tetramethylrhodamine ethyl ether probe specifically accumulating in energized mitochondria significantly decreased in renal tubular epithelium after 40-min thermal ischemia, while fluorescence of dichlorodihydrofluorescein and diaminofluorescein probes in the same structures increased under these conditions, which attests to increased generation of ROS and NO, respectively. These forms were generated predominantly in mitochondria of tubular epitheliocytes. Hypoxic preconditioning (a series of sessions of breathing hypoxic mixture) preserved functional activity of mitochondria and prevented activation of ROS and NO generation. Ischemic preconditioning of the kidney consisting of three preliminary episodes of vascular clamping (5 min with 5 min reperfusion periods) also increased the percentage of functionally active mitochondria and prevented activation of NO synthesis without appreciably modifying ROS production. Both protective methods significantly reduced the severity of postischemic dysfunction of the kidney.
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Authors | V I Kirpatovskii, A V Kazachenko, E Yu Plotnikov, T A Kon'kova, V V Drozhzheva, D B Zorov |
Journal | Bulletin of experimental biology and medicine
(Bull Exp Biol Med)
Vol. 143
Issue 1
Pg. 105-9
(Jan 2007)
ISSN: 0007-4888 [Print] United States |
PMID | 18019025
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Reactive Oxygen Species
- Nitric Oxide
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Topics |
- Animals
- Hypoxia
(metabolism, physiopathology)
- Ischemia
(metabolism, physiopathology, prevention & control)
- Ischemic Preconditioning
- Kidney
(blood supply, physiopathology, ultrastructure)
- Kidney Tubules
(metabolism, ultrastructure)
- Male
- Microscopy, Confocal
- Mitochondria
(metabolism)
- Nitric Oxide
(metabolism)
- Rats
- Reactive Oxygen Species
(metabolism)
- Urothelium
(metabolism, ultrastructure)
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