[Orotate phosphoribosyltransferase levels in gastric carcinoma and normal gastric mucosa - correlation with thymidylate synthase and dihydropyrimidine dehydrogenase levels and the changes after neoadjuvant chemotherapy].

Abstract

Orotate phosphoribosyltransferase (OPRT, EC 2.4.2.10), a key enzyme that catalyzes one of the primary first-step phosphorylation processes of fluoropyrimidine, has recently been recognized as an important factor that primarily determines the anticancer effects of S-1. The OPRT levels were examined in 97 gastric carcinoma tissues and 65 normal gastric mucosa tissues obtained from patients with gastric carcinoma using a newly-developed enzyme-linked immunosorbent assay. Correlations with thymidylate synthase and dihydropyrimidine dehydrogenase levels and the effects of neoadjuvant chemotherapy were evaluated. The OPRT level in gastric carcinoma tissue was significantly higher than that in normal gastric mucosa. There was no correlation of OPRT level with either TS or DPD levels. There was no correlation of OPRT level between those in gastric carcinoma and those in normal gastric mucosa simultaneously obtained from identical patients. The OPRT levels in patients who underwent neoadjuvant chemotherapy were not different from those without neoadjuvant chemotherapy. These results suggest that activation of fluoropyrimidine mainly occurs in carcinoma tissues and the OPRT levels in carcinoma tissues were not influenced by neoadjuvant chemotherapy with fluoropyrimidine.

• Authors: Yoichi Sakurai, Shinpei Furuta, Risaburo Sunagawa, Shuhei Tonomura, Kazuki Inaba, Mitsutaka Shoji, Yasuko Nakamura, Jun Isogaki, Yoshiyuki Komori, Ichiro Uyama, Shingo Kamoshida, Yutaka Tsutsumi
• Journal: Gan to kagaku ryoho. Cancer & chemotherapy (Gan To Kagaku Ryoho) Vol. 34 Issue 10 Pg. 1581-7 (Oct 2007) ISSN: 0385-0684 [Print] Japan
• PMID: 17940372 (Publication Type: English Abstract, Journal Article)

Chemical References

• Dihydrouracil Dehydrogenase (NADP)
• Thymidylate Synthase
• Orotate Phosphoribosyltransferase

Topics

• Aged
• Chemotherapy, Adjuvant
• Dihydrouracil Dehydrogenase (NADP) (analysis)
• Enzyme Activation
• Enzyme-Linked Immunosorbent Assay
• Female
• Gastric Mucosa (enzymology)
• Humans
• Immunohistochemistry
• Male
• Neoadjuvant Therapy
• Orotate Phosphoribosyltransferase (analysis)
• Stomach Neoplasms (enzymology)
• Thymidylate Synthase (analysis)