Platelet activating factor as a proinflammatory mediator in acetic-induced colitis in the rat.

Abstract

Platelet activating factor (PAF) induces neutrophilia and produces a variety of gastrointestinal lesions. The role of PAF as a proinflammatory mediator was examined by measuring the production of PAF and the efficacy of the PAF antagonists WEB 2086 and Ro 24-0238 in acetic acid (HOAc)-induced colitis. PAF levels within the colonic mucosa, as measured by radioimmunoassay, increased from 259 +/- 119 ng/mg in control tissue, to 616 +/- 266 ng/mg in HOAc inflamed tissue. The accumulation of neutrophils within the mucosa was decreased by 53 +/- 10% by pretreatment with 3 mg/kg WEB 2086, and by 43 +/- 11% by 3 mg/kg Ro 24-0238. PAF-induced neutrophilia had no effect on the severity of HOAc-induced colitis, therefore, PAF my be involved in sustaining the chronic inflammation of colitis.

• Authors: P C Will, T K Thomas, L Iverson, D Buckman, W Weis, C Wilson, A Srivastava
• Journal: Agents and actions (Agents Actions) Vol. 34 Issue 1-2 Pg. 181-4 (Sep 1991) ISSN: 0065-4299 [Print] Switzerland
• PMID: 1793026 (Publication Type: Journal Article)

Chemical References

• Acetates
• Azepines
• Platelet Activating Factor
• Polyunsaturated Alkamides
• Pyridines
• Triazoles
• WEB 2086
• Ro 24-0238

Topics

• Acetates
• Animals
• Azepines (pharmacology)
• Colitis (chemically induced, metabolism)
• Inflammation (metabolism)
• Platelet Activating Factor (antagonists & inhibitors, pharmacology, physiology)
• Polyunsaturated Alkamides
• Pyridines
• Rats
• Rats, Inbred Strains
• Triazoles (pharmacology)