Fluorescent probe ABM for screening gastrointestinal patient's immune state.

Abstract

The fluorescent probe-aminoderivative of benzanthrone, ABM (developed at Riga Technical University, Riga, Latvia) was used to characterize the membranes of lymphocytes of cancer patients: 46 patients with gastrointestinal diseases, 13 patients having different primary localizations with massive metastases and intoxication. Patients were divided into three groups: (1) with decreased fluorescence intensity, (2) normal fluorescence intensity, (3) increased fluorescence intensity. The lymphocytes distribution among subsets differed between groups, in correspondence to the level of fluorescence intensity. Surgical treatment affected the main immunological parameters and elevated the functional activity of lymphocytes. In the advanced tumors group, fluorescence intensity correlates with the survival rate. Results suggest that determination of lymphocytes functional activity by ABM can aid evaluation of the immune status in cancer patients.

• Authors: Inta Kalnina, Laura Klimkane, Elena Kirilova, Maija Malda Toma, Gunta Kizane, Imants Meirovics
• Journal: Journal of fluorescence (J Fluoresc) Vol. 17 Issue 6 Pg. 619-25 (Nov 2007) ISSN: 1053-0509 [Print] Netherlands
• PMID: 17926122 (Publication Type: Journal Article)

Chemical References

• Benz(a)Anthracenes
• Fluorescent Dyes

Topics

• Benz(a)Anthracenes (chemistry)
• Cell Membrane (immunology)
• Fluorescent Dyes (chemistry)
• Gastrointestinal Neoplasms (immunology, therapy)
• Humans
• Lymphocytes (immunology)
• Spectrometry, Fluorescence