Impairment of the host's antibacterial resistance by norepinephrine activated neutrophils.

Abstract

The susceptibility of mice to infectious complications is dramatically increased in an accompaniment with systemic inflammatory response syndrome (SIRS). Polymorphonuclear neutrophils with immunosuppressive ability (PMN-II) that appear in response to SIRS have been classified as one of the cells responsible for the increased susceptibility of mice with SIRS (SIRS mice) to sepsis induced by cecal-ligation and puncture (CLP). Since a high level of norepinephrine (NE) is demonstrated in the plasma of SIRS mice, in the present study, the role of NE on the appearance of PMN-II in SIRS mice was studied. Similar to SIRS mice, normal mice became susceptible to CLP-induced infectious complications after inoculation with NE-treated PMN. CCL2 and IL-10 (biomarkers for PMN-II) were equally produced by PMN-II prepared from SIRS mice and NE-treated PMN. However, CCL3 and IL-12 (biomarkers for immunostimulatory PMN, PMN-I) were not detected in culture fluids from either PMN preparation. These results indicate that NE mass-produced in association with SIRS development plays a role on the generation of PMN-II and the appearing PMN-II are responsible, in part, for increased susceptibility of SIRS mice to CLP-induced infectious complications.

• Authors: Yasuhiro Tsuda, Makiko Kobayashi, David N Herndon, Fujio Suzuki
• Journal: Burns : journal of the International Society for Burn Injuries (Burns) Vol. 34 Issue 4 Pg. 460-6 (Jun 2008) ISSN: 0305-4179 [Print] Netherlands
• PMID: 17913370 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CCL2
• Chemokine CCL3
• Interleukin-10
• Norepinephrine

Topics

• Animals
• Burns (immunology)
• Cecum (surgery)
• Chemokine CCL2 (biosynthesis)
• Chemokine CCL3 (biosynthesis)
• Drug Resistance, Bacterial (immunology)
• Interleukin-10 (biosynthesis)
• Ligation
• Male
• Mice
• Mice, Inbred BALB C
• Neutrophil Activation (immunology)
• Neutrophils (drug effects, immunology)
• Norepinephrine (metabolism, pharmacology, physiology)
• Pancreatitis (immunology)
• Punctures
• Systemic Inflammatory Response Syndrome (immunology)