Effect of plaunotol in combination with clarithromycin against clarithromycin-resistant Helicobacter pylori in vitro and in vivo.

Abstract

OBJECTIVES:

Recently, there has been a decrease in the eradication rate of Helicobacter pylori due to the increase in antibiotic resistance of this bacterium. Plaunotol, a cytoprotective anti-ulcer agent, exhibits antibacterial activity against H. pylori. The purpose of the present study was to investigate the effect of plaunotol in combination with clarithromycin against clarithromycin-resistant H. pylori clinical isolates.

METHODS AND RESULTS:

In the chequerboard titration method, the combination of plaunotol and clarithromycin showed a synergistic effect against 67% (10/15) clarithromycin-resistant strains and an additive effect against the other strains. No indifferent and antagonistic effects were observed against any of the strains tested. In a gastritis model of Mongolian gerbils infected with clarithromycin-resistant H. pylori, the plaunotol (40 mg/kg) and clarithromycin (66.6 mg/kg) combination exhibited synergistic effects; however, neither plaunotol nor clarithromycin alone showed bactericidal effects.

CONCLUSIONS:

These results suggest that plaunotol may play a useful role in combination with anti-H. pylori drugs in the treatment of diseases associated with clarithromycin-resistant H. pylori.

• Authors: Makoto Sasaki, Tsutomu Mizoshita, Takashi Mizushima, Harumi Inoue, Takeshi Kamiya, Hiromi Kataoka, Naotaka Ogaswara, Tsuneya Wada, Eiji Kubota, Yoshinori Mori, Takaya Shimura, Hirokazu Hirata, Kenji Ando, Yasuyuki Okamoto, Hirotaka Ohara, Haruhisa Nakao, Takashi Joh
• Journal: The Journal of antimicrobial chemotherapy (J Antimicrob Chemother) Vol. 60 Issue 5 Pg. 1060-3 (Nov 2007) ISSN: 0305-7453 [Print] England
• PMID: 17827146 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Diterpenes
• Fatty Alcohols
• Clarithromycin
• plaunotol

Topics

• Animals
• Anti-Bacterial Agents (administration & dosage, therapeutic use)
• Clarithromycin (administration & dosage, therapeutic use)
• Diterpenes
• Drug Resistance, Bacterial
• Drug Therapy, Combination
• Fatty Alcohols (administration & dosage, therapeutic use)
• Genetic Variation
• Gerbillinae
• Helicobacter Infections (drug therapy)
• Helicobacter pylori (drug effects, genetics)
• Male
• Microbial Sensitivity Tests
• Specific Pathogen-Free Organisms