[Study of inhibitory effect of extracts from Actinidia arguta on human carcinoma of esophagus cells].

Abstract

OBJECTIVE:

To study the inhibitory effect of extracts from Actinidia arguta by n-butyl alcohol on human carcinoma of esophagus cells (Eca-109) and Its mechanism.

METHODS:

MTT colonmetric assay was used to examine the growth inhibitory of concentration-effect (1 microg/ml, 10 microg/ml,100 microg/ml) and time-effect (24 h, 48 h, 72 h) of extracts from Actinidia arguta by n-butyl alcohol on Eca-109 cells. TUNEL test were performed to observe the apoptosis induced by the extracts (1 microg/ml, 10 microg/ml, 100 microg/ml) on Eca-109 cells.

RESULTS:

The inhibitory effect of the extracts on Eca-109 cells increased in a dose-time Manner and the highest rate of inhibition was 87.2%. The extracts could significantly induce apoptosis of Eca-109 cells, but in control group, apoptosis wasn't observed.

CONCLUSION:

The extracts from Actinidia arguta by n-butyl alcohol have good inhibitory effect on Eca-109 cells.

• Authors: Li Zhang, Hong-Li Guo, Lin Tian, Shu-Fen Cao, Chang-Hai Du
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 30 Issue 5 Pg. 564-6 (May 2007) ISSN: 1001-4454 [Print] China
• PMID: 17727063 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Drugs, Chinese Herbal

Topics

• Actinidia (chemistry)
• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacology)
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (administration & dosage, pharmacology)
• Esophageal Neoplasms (pathology)
• Humans
• Plant Roots (chemistry)
• Plants, Medicinal (chemistry)
• Time Factors