[Serum hyaluronic acid, tumor necrosis factor -alpha, vascular endothelial growth factor, NO, and Se levels in adult patients with Kashin-Beck disease].

Abstract

OBJECTIVE:

To investigate the association of serum levels of hyaluronic acid (HA), tumor necrosis factor-alpha (TNF-alpha), vascular endothelial growth factor (VEGF), NO, and Se with the clinical manifestations in adult patients with Kashin-Beck disease (KBD).

METHODS:

Total 216 adults were selected for KBD screening from the KBD-prevalent areas in Yongshou county and the non-KBD areas of Chang'an county, Xi'an city, ShaanXi Province. According to the National Diagnostic Criteria of Kashin-Beck Disease in China, the diagnoses of KBD was established in 25 adult patients (11 men and 14 women, average age of 47.88+/-11.16 years), and 20 healthy control subjects from the KBD areas (8 men and 12 women, average age of 47.85+/-12.05 years) and 20 from the non-KBD areas (8 men and 12 women, average age of 47.45+/-11.24 years) were also selected to serve as controls. There was no significant difference in the average age and gender distribution between the 3 groups. The serum levels of HA, TNF-alpha, VEGF, NO and Se were measured by enzyme-linked immunosorbent assay, nitrate reductase method and griphite furnace atomic absorption spectrometry.

RESULTS:

Serum NO level was significantly higher in KBD group (41.7+/-21.89 micromol/L) than in the health controls from KBD areas (17.1+/-13.01 micromol/L) and non-KBD areas (17.58+/-11.48 micromol/l, F=13.11, df=2, P<0.001). Serum TNF-alpha level in KBD group (32.7+/-3.55 pg/ml) was significantly higher than that in the control subjects from the non-KBD areas (30.95+/-2.22 pg/ml, F=3.672, df=2, P=0.031), but similar with the control subjects from the KBD areas (32.7+/-3.55 pg/ml). Serum TNF-alpha and NO levels were identified as the indices that differed between adult KBD patients and the controls from both KBD and non-KBD areas by differential analysis (the function of differentiation was 0.062xNO+0.173xTNF -7.218).

CONCLUSION:

Serum TNF-alpha and NO levels are significantly increased in adult KBD patients and are associated with the clinical manifestations of KBD.

• Authors: Xiao-ying Li, Xiong Guo, Li-xin Wang, Dong Geng, Long-li Kang, Shuang Wang, Zhi-fang Wang, Qi-sheng Gu
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 27 Issue 7 Pg. 941-4 (Jul 2007) ISSN: 1673-4254 [Print] China
• PMID: 17666321 (Publication Type: English Abstract, Journal Article)

Chemical References

• Tumor Necrosis Factor-alpha
• Vascular Endothelial Growth Factor A
• Nitric Oxide
• Hyaluronic Acid
• Selenium

Topics

• Adult
• Aged
• Bone Diseases (blood)
• Case-Control Studies
• Female
• Humans
• Hyaluronic Acid (blood)
• Male
• Middle Aged
• Nitric Oxide (blood)
• Selenium (blood)
• Tumor Necrosis Factor-alpha (blood)
• Vascular Endothelial Growth Factor A (blood)