Monocyte chemoattractant protein-1 or macrophage inflammatory protein-1alpha deficiency does not affect angiotensin II-induced intimal hyperplasia in carotid artery ligation model.

Abstract

BACKGROUND:

Angiotensin II (Ang II) promotes atherosclerotic vascular diseases, in which proinflammatory and proliferative effects play a major pathogenic role. Ang II up-regulates chemokines, such as monocyte chemoattractant protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha, which are important pro-inflammatory factors mediating infiltration of inflammatory cells into atherosclerotic lesion. The aim of the present study was to determine whether the presence of MCP-1 or MIP-1alpha is essential in Ang II-induced intimal hyperplasia in the carotid artery ligation model.

METHODS:

Six-month-old male C57BL/6-, MCP-1-, or MIP-1alpha-deficient mice underwent ligation of the common left carotid artery and were randomly assigned to receive either vehicle or Ang II (1.4 mg kg(-1) day(-1)) via a subcutaneously implanted osmotic infusion pump (model 2004, Alzet) for 4 weeks.

RESULTS:

Ang II not only increased MCP-1 and MIP-1alpha production but also enhanced neo-intimal formation, media thickness, and adventitia development in the ligated carotid arteries in C57BL/6 mice. However, MCP-1 or MIP-1alpha deficiency failed to affect intimal hyperplasia in vascular remodeling.

CONCLUSION:

These results indicate that MCP-1 or MIP-1alpha may not be essential in mediating the proliferative effects of Ang II, a major pathological changes in intimal hyperplasia in the carotid artery ligation model.

• Authors: Le-Ning Zhang, Valdeci da Cunha, Baby Martin-McNulty, John Rutledge, Ronald Vergona, Mark E Sullivan, Yi-Xin Jim Wang
• Journal: Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology (Cardiovasc Pathol) 2007 Jul-Aug Vol. 16 Issue 4 Pg. 231-6 ISSN: 1054-8807 [Print] United States
• PMID: 17637431 (Publication Type: Journal Article)

Chemical References

• Ccl2 protein, mouse
• Chemokine CCL2
• Chemokine CCL3
• Chemokine CCL4
• Macrophage Inflammatory Proteins
• Angiotensin II

Topics

• Angiotensin II (metabolism)
• Animals
• Carotid Arteries (metabolism, pathology)
• Carotid Artery Injuries (metabolism, pathology)
• Chemokine CCL2 (metabolism)
• Chemokine CCL3
• Chemokine CCL4
• Hyperplasia
• Immunohistochemistry
• Ligation
• Macrophage Inflammatory Proteins (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Tunica Intima (metabolism, pathology)