Increased Cyfra 21-1 concentration in saliva from primary oral squamous cell carcinoma patients.

Abstract

BACKGROUND:

Saliva is the body fluid in the oral cavity and contacts directly with oral mucosa. As a detective media, it is acceptable and non-traumatic. Cyfra 21-1, being the soluble fragment of cytokeratin 19(CK19), correlates well with oral squamous cell carcinoma (OSCC).

OBJECTIVE:

To investigate the saliva Cyfra 21-1 concentrations in OSCC patients and healthy persons, and the correlation between the Cyfra 21-1 concentration in saliva and the CK19 expression in tissue from OSCC patients.

DESIGN:

Saliva Cyfra 21-1 concentration was detected by ELISA in 30 OSCC patients and 30 healthy persons; CK19 protein expression and CK19 mRNA level were, respectively, detected by immunohistochemistry and fluorescent real-time RT-PCR in cancerous and paracancerous tissues from 33 OSCC patients.

RESULTS:

Saliva Cyfra 21-1 concentration in OSCC patients (85.95+/-78.00 microg/L) was significantly higher than that in healthy persons (42.27+/-40.84 microg/L) (P=0.009); it was also significantly higher in the patients suffering later tumour recurrence (130.95+/-66.38 microg/L) than that in the patients without tumour recurrence (74.84+/-63.45 microg/L) (P=0.023). CK19 protein expression increased significantly in OSCC tissues (P<0.001) with positive rate of 90.9%, CK19 mRNA level in cancerous tissues was 2.21 folds higher than that in paracancerous tissues (P=0.020); significant correlation was found between tissue CK19 protein expression and tissue CK19 mRNA level (P=0.003), and great correlation was found between tissue CK19 protein expression and saliva Cyfra 21-1 concentration (P=0.051).

CONCLUSIONS:

The increased CK19 expression in OSCC tissues plays an important role in the increase of saliva Cyfra 21-1 concentration. Potential clinical value of saliva Cyfra 21-1 detection is suggested for OSCC. Further studies are encouraged to reveal the real diagnostic and prognostic value of detecting saliva Cyfra 21-1 concentration for OSCC.

• Authors: Lai-ping Zhong, Chen-ping Zhang, Jia-wei Zheng, Jiang Li, Wan-tao Chen, Zhi-yuan Zhang
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 52 Issue 11 Pg. 1079-87 (Nov 2007) ISSN: 0003-9969 [Print] England
• PMID: 17612501 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Keratin-19
• RNA, Messenger
• antigen CYFRA21.1
• Keratins

Topics

• Adult
• Aged
• Aged, 80 and over
• Antigens, Neoplasm (analysis)
• Biomarkers, Tumor (analysis)
• Carcinoma, Squamous Cell (immunology, mortality, pathology)
• Case-Control Studies
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Humans
• Immunohistochemistry
• Keratin-19 (analysis, genetics)
• Keratins (analysis)
• Male
• Middle Aged
• Mouth Neoplasms (immunology, mortality, pathology)
• Neoplasm Staging
• Precancerous Conditions (immunology, mortality, pathology)
• RNA, Messenger (analysis)
• ROC Curve
• Reverse Transcriptase Polymerase Chain Reaction
• Saliva (immunology)
• Statistics, Nonparametric
• Survival Rate