Proinflammatory cytokines mediating burn-injury pain.

Abstract

Thermal burns induce pain at the site of injury, mechanical hyperalgesia, associated with a complex time-dependent inflammatory response. To determine the contribution of inflammatory mediators to burn injury-induced mechanical hyperalgesia, we measured dynamic changes in the levels of three potent hyperalgesic cytokines, interleukin IL-1 beta, IL-6, and tumor necrosis factor-alpha (TNFalpha), in skin of the rat, following a partial-thickness burn injury. Only IL-6 demonstrated a sustained increase ipsilateral but not contralateral to the burn, correlating with the prolonged ipsilateral mechanical hyperalgesia. Spinal intrathecal injection of oligodeoxynucleotides antisense for gp130, a receptor subunit shared by members of the IL-6 family of cytokines, attenuated both burn- and intradermal IL-6-induced hyperalgesia, as did intradermal injection of anti-IL-6 function blocking antibodies. These studies suggest that IL-6 is an important mediator of burn-injury pain.

• Authors: Gretchen J Summer, Edgar Alfonso Romero-Sandoval, Oliver Bogen, Olayinka A Dina, Sachia G Khasar, Jon D Levine
• Journal: Pain (Pain) Vol. 135 Issue 1-2 Pg. 98-107 (Mar 2008) ISSN: 1872-6623 [Electronic] United States
• PMID: 17590515 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Cytokines
• Interleukin-6
• Morpholines
• Oligodeoxyribonucleotides, Antisense
• 2-phenyl-5,5-dimethyltetrahydro-1,4-oxazine

Topics

• Analysis of Variance
• Animals
• Burns (complications)
• Cytokines (metabolism)
• Disease Models, Animal
• Functional Laterality
• Hyperalgesia (drug therapy, etiology, immunology, metabolism)
• Injections, Spinal (methods)
• Interleukin-6 (metabolism)
• Male
• Morpholines (chemistry)
• Oligodeoxyribonucleotides, Antisense (therapeutic use)
• Pain Measurement
• Pain Threshold (drug effects)
• Rats
• Rats, Sprague-Dawley
• Skin (drug effects, metabolism, pathology)