Analysis of sofalcone in human plasma by high performance liquid chromatography.

Abstract

A simple, rapid, sensitive and reliable high performance liquid chromatography (HPLC) method for the determination of the anti-ulcer drug sofalcone in human plasma was developed. Plasma was extracted with ethyl acetate under acidic conditions and sofalcone was determined by HPLC using a C18 column and (methanol-0.1% formic acid aqueous 80:20) mobile phase. The linear calibration curves of sofalcone in human plasma were obtained over the concentration range of 0.01-5.0 microg/ml. The lower limit of quantitation (LLOQ) was 10 ng/ml in human plasma. The precision measured for plasma was within 15%. Extraction recovery was over 85% in blood. The method was successfully applied to the identification and quantification of sofalcone in pharmacokinetic studies.

• Authors: Aidong Wen, Zhipeng Wang, Taijun Hang, Yanyan Jia, Tingting Zhang, Yin Wu, Xuan Gao, Zhifu Yang
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 856 Issue 1-2 Pg. 348-52 (Sep 01 2007) ISSN: 1570-0232 [Print] Netherlands
• PMID: 17586104 (Publication Type: Journal Article)

Chemical References

• Anti-Ulcer Agents
• Chalcones
• sofalcone

Topics

• Anti-Ulcer Agents (blood, pharmacokinetics)
• Calibration
• Chalcones (blood, pharmacokinetics)
• Chromatography, High Pressure Liquid (methods)
• Humans
• Quality Control
• Sensitivity and Specificity