Analysis of sofalcone in human plasma by high performance liquid chromatography.

A simple, rapid, sensitive and reliable high performance liquid chromatography (HPLC) method for the determination of the anti-ulcer drug sofalcone in human plasma was developed. Plasma was extracted with ethyl acetate under acidic conditions and sofalcone was determined by HPLC using a C18 column and (methanol-0.1% formic acid aqueous 80:20) mobile phase. The linear calibration curves of sofalcone in human plasma were obtained over the concentration range of 0.01-5.0 microg/ml. The lower limit of quantitation (LLOQ) was 10 ng/ml in human plasma. The precision measured for plasma was within 15%. Extraction recovery was over 85% in blood. The method was successfully applied to the identification and quantification of sofalcone in pharmacokinetic studies.
AuthorsAidong Wen, Zhipeng Wang, Taijun Hang, Yanyan Jia, Tingting Zhang, Yin Wu, Xuan Gao, Zhifu Yang
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 856 Issue 1-2 Pg. 348-52 (Sep 1 2007) ISSN: 1570-0232 [Print] Netherlands
PMID17586104 (Publication Type: Journal Article)
Chemical References
  • Anti-Ulcer Agents
  • Chalcones
  • sofalcone
  • Anti-Ulcer Agents (blood, pharmacokinetics)
  • Calibration
  • Chalcones (blood, pharmacokinetics)
  • Chromatography, High Pressure Liquid (methods)
  • Humans
  • Quality Control
  • Sensitivity and Specificity

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