Abstract |
Bordetella pertussis has a distinctive cell wall lipooligosaccharide (LOS) that is released from the bacterium during bacterial division and killing. LOS directly participates in host-bacterial interactions, in particular influencing the dendritic cells' (DC) immune regulatory ability. We analyze LOS mediated toll-like receptor (TLR) activation and dissect the role played by LOS on human monocyte-derived (MD)DC functions and polarization of the host T cell response. LOS activates TLR4-dependent signaling and induces mature MDDC able to secrete IL-10. LOS-matured MDDC enhance allogeneic presentation and skew T helper (Th) cell polarization towards a Th2 phenotype. LOS protects MDDC from undergoing apoptosis, prolonging their longevity and their functions. Compared to Escherichia coli lipopolysaccharide (LPS), the classical DC maturation stimulus, LOS was a less efficient inducer of TLR4 signaling, MDDC maturation, IL-10 secretion and allogeneic T cell proliferation and it was not able to induce IL-12p70 production in MDDC. However, the MDDC apoptosis protection exerted by LOS and LPS were comparable. In conclusion, LOS treated MDDC are able to perform antigen presentation in a context that promotes licensing of Th2 effectors. Considering these properties, the use of LOS in the formulation of acellular pertussis vaccines to potentiate protective and adjuvant capacity should be taken into consideration.
|
Authors | Giorgio Fedele, Ignacio Celestino, Fabiana Spensieri, Loredana Frasca, Maria Nasso, Mineo Watanabe, Maria Elena Remoli, Eliana Marina Coccia, Fabio Altieri, Clara Maria Ausiello |
Journal | Microbes and infection
(Microbes Infect)
Vol. 9
Issue 7
Pg. 855-63
(Jun 2007)
ISSN: 1286-4579 [Print] France |
PMID | 17533149
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Lipopolysaccharides
- TLR4 protein, human
- Toll-Like Receptor 4
- Virulence Factors, Bordetella
- pertussis endotoxin
- Interleukin-10
- RNA
|
Topics |
- Apoptosis
(immunology)
- Bordetella pertussis
(immunology)
- Cell Proliferation
- Dendritic Cells
(immunology, microbiology)
- Flow Cytometry
- Humans
- Immunophenotyping
- Interleukin-10
(genetics, immunology)
- Lipopolysaccharides
(immunology, pharmacology)
- RNA
(chemistry, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction
(immunology)
- T-Lymphocytes
(immunology, microbiology)
- Th2 Cells
(immunology, microbiology)
- Toll-Like Receptor 4
(immunology)
- Virulence Factors, Bordetella
(immunology, pharmacology)
- Whooping Cough
(immunology, microbiology)
|