Abstract | OBJECTIVE: DESIGN: Ishikawa cells were cultured to 80% confluence, in vitro. After 24h incubation in serum-free media, 1.0, 0.1 and 0.01 microM of 17beta-estradiol, medroxyprogesterone acetate, tibolone, 3alpha-hydroxytibolone, 3beta-hydroxytibolone, and Delta4-tibolone were added to the Ishikawa cells. The cells plus steroids were then incubated for a further 24h. Total RNA was extracted from control and treated Ishikawa cells. After reverse transcription, Angiopoietin-1, Tie-2, tumor necrosis factor-alpha, and beta-actin cDNAs were amplified in a polymerase chain reaction spiked with 33p-dCTP. Relative abundance of Angiopoietin-1, Tie-2, and tumor necrosis factor-alpha mRNA was measured by scintillation spectroscopy. RESULTS: CONCLUSIONS:
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Authors | Sebastian Mirkin, David F Archer |
Journal | Maturitas
(Maturitas)
Vol. 57
Issue 4
Pg. 338-46
(Aug 20 2007)
ISSN: 0378-5122 [Print] Ireland |
PMID | 17478063
(Publication Type: Journal Article)
|
Chemical References |
- Angiopoietin-1
- Contraceptive Agents, Female
- Estrogen Receptor Modulators
- Norpregnenes
- RNA, Messenger
- Receptors, Estrogen
- Receptors, Progesterone
- Tumor Necrosis Factor-alpha
- Estradiol
- Medroxyprogesterone Acetate
- Receptor, TIE-2
- tibolone
|
Topics |
- Adenocarcinoma
(metabolism, pathology)
- Angiopoietin-1
(genetics, metabolism)
- Cell Line, Tumor
- Contraceptive Agents, Female
(pharmacology)
- Endometrial Neoplasms
(metabolism, pathology)
- Endometrium
(drug effects, metabolism, pathology)
- Estradiol
(pharmacology)
- Estrogen Receptor Modulators
(metabolism, pharmacology)
- Female
- Humans
- Medroxyprogesterone Acetate
(pharmacology)
- Norpregnenes
(metabolism, pharmacology)
- RNA, Messenger
(genetics, metabolism)
- Receptor, TIE-2
(genetics, metabolism)
- Receptors, Estrogen
(genetics, metabolism)
- Receptors, Progesterone
(genetics, metabolism)
- Tumor Necrosis Factor-alpha
(genetics, metabolism)
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