Role of cyclin-dependent kinase 5 in capsaicin-induced cough.

Abstract

The role of cyclin-dependent kinase 5 (Cdk5) in the capsaicin-induced cough reflex was examined in mice. Pretreatment with inhaled roscovitine, a selective Cdk5 inhibitor, at concentrations of 0.3 to 3 mM inhibited the number of capsaicin-induced coughs in a concentration-dependent manner. Pretreatment with inhaled roscovitine, at a concentration of 3 mM also slightly but significantly inhibited the number of citric acid-induced coughs. The number of capsaicin-induced coughs was significantly reduced when C-fibers were desensitized by the pretreatment with capsaicin. The number of citric acid-induced coughs was slightly but significantly reduced in capsaicin-pretreated mice as compared with that in naive mice. Although the inhalation of roscovitine at a concentration of 3 mM significantly reduced the number of citric acid-induced coughs in naive mice to the level observed in capsaicin-pretreated mice, roscovitine had no effect on the number of citric acid-induced coughs in capsaicin-pretreated mice. These results suggest that Cdk5-dependent factors are involved in C-fiber-mediated cough signaling.

• Authors: Junzo Kamei, Shun-suke Hayashi, Yoshiki Takahashi, Chihiro Nozaki
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 566 Issue 1-3 Pg. 181-4 (Jul 02 2007) ISSN: 0014-2999 [Print] Netherlands
• PMID: 17459370 (Publication Type: Journal Article)

Chemical References

• Antitussive Agents
• Protein Kinase Inhibitors
• Purines
• Roscovitine
• Citric Acid
• Cyclin-Dependent Kinase 5
• Capsaicin

Topics

• Animals
• Antitussive Agents (pharmacology)
• Capsaicin
• Citric Acid
• Cough (chemically induced, drug therapy)
• Cyclin-Dependent Kinase 5 (antagonists & inhibitors)
• Male
• Mice
• Mice, Inbred ICR
• Protein Kinase Inhibitors (pharmacology)
• Purines (pharmacology)
• Roscovitine