Effect of resuscitation with 21% oxygen and 100% oxygen on NMDA receptor binding characteristics following asphyxia in newborn piglets.

Abstract

The present study investigated the effect of reventilation with 21% and 100% oxygen following asphyxia in newborn piglets on NMDA receptor binding characteristics, Na(+), K(+)-ATPase activity, and lipid peroxidation. After achieving a heart rate less than 60 beats per minute, asphyxiated piglets were reventilated with 21% oxygen or 100% oxygen. (3)[H]MK-801 binding showed the Bmax in the 21% and 100% groups to be 1.53 +/- 0.43 and 1.42 +/- 0.35 pmol/mg protein (p = ns). Values for Kd were 4.56 +/- 1.29 and 4.17 +/- 1.05 nM (p = ns). Na(+), K(+)-ATPase activity in the 21% and 100% groups were 23.5 +/- 0.9 and 24.4 +/- 3.9 micromol Pi/mg protein/h (p = ns). Conjugated dienes (0.05 +/- 0.02 vs. 0.07 +/- 0.03 micromol/g brain) and fluorescent compounds (0.54 +/- 0.05 vs. 0.78 +/- 0.19 microg quinine sulfate/g brain), were similar in both groups (p = ns). Though lipid peroxidation products trended higher in the 100% group, these data show that NMDA receptor binding and Na(+), K(+)-ATPase activity were similar following reventilation with 21% or 100% oxygen after a single episode of mild asphyxia.

• Authors: David Joseph Hoffman, Eric Lombardini, Om Prakash Mishra, Maria Delivoria-Papadopoulos
• Journal: Neurochemical research (Neurochem Res) Vol. 32 Issue 8 Pg. 1322-8 (Aug 2007) ISSN: 0364-3190 [Print] United States
• PMID: 17401653 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Excitatory Amino Acid Antagonists
• Receptors, N-Methyl-D-Aspartate
• Dizocilpine Maleate
• Adenosine Triphosphate
• Sodium-Potassium-Exchanging ATPase
• Oxygen

Topics

• Adenosine Triphosphate (metabolism)
• Animals
• Animals, Newborn
• Asphyxia (therapy)
• Brain (cytology, metabolism)
• Dizocilpine Maleate (metabolism)
• Excitatory Amino Acid Antagonists (metabolism)
• Humans
• Infant, Newborn
• Oxygen (metabolism)
• Oxygen Inhalation Therapy
• Receptors, N-Methyl-D-Aspartate (metabolism)
• Resuscitation
• Sodium-Potassium-Exchanging ATPase (metabolism)