Apoptosis repressor with caspase recruitment domain (ARC) inhibits myogenic differentiation.

Abstract

Apoptosis repressor with caspase recruitment domain (ARC), an anti-apoptotic protein, is highly expressed in differentiated heart and skeletal muscle. Apoptosis and differentiation share numerous common pathways; therefore, we examined the impact of ARC on H9c2-myoblast differentiation. We demonstrate that ARC expression levels increase and stabilize upon differentiation. ARC-overexpression in pre-differentiated H9c2-cells suppresses differentiation; indicated by increased myotube formation, nuclear fusion and expression of the differentiation markers myogenin and troponin-T. ARC-overexpression inhibited myoblast differentiation associated caspase-3 activation, suggesting ARC inhibits myogenic differentiation through caspase inhibition. In summary, we show a novel role for ARC in the regulation of muscle differentiation.

• Authors: Arwen L Hunter, Jingchun Zhang, Shirley C Chen, Xiaoning Si, Brian Wong, Daryoush Ekhterae, Honglin Luo, David J Granville
• Journal: FEBS letters (FEBS Lett) Vol. 581 Issue 5 Pg. 879-84 (Mar 06 2007) ISSN: 0014-5793 [Print] England
• PMID: 17292893 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apoptosis Regulatory Proteins
• Muscle Proteins
• Nol3 protein, rat
• Recombinant Proteins
• Casp3 protein, rat
• Caspase 3

Topics

• Animals
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Caspase 3 (metabolism)
• Cell Differentiation (physiology)
• Cell Line
• Enzyme Activation
• Gene Expression
• Muscle Development (physiology)
• Muscle Proteins (genetics, metabolism)
• Myoblasts, Cardiac (cytology, metabolism)
• Rats
• Recombinant Proteins (genetics, metabolism)
• Transfection