Abstract | BACKGROUND: MATERIALS AND METHODS:
cDNA microarrays were used to profile feature genes in 5637 and T24 cells before and after treatment with gefitinib. PCR-based direct sequencing and Western blot analysis were performed to examine the mutation status and protein levels of EGFR in the cell lines. RESULTS:
Gefitinib significantly inhibited the proliferation of 5637 cells, while showing little inhibitory effect on T24 cells. Theses effects were independent of the mutation status and protein levels of EGFR. cDNA microarray analysis identified 15 feature genes classified as a cell cycle, apoptotic pathway and transcription. Notably, levels of expression of the cell invasion-related genes, YY1 and E-cadherin, were increased in 5637 cells sensitive to gefitinib. CONCLUSION:
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Authors | Ryo Inoue, Hideyasu Matsuyama, Seiji Yano, Yoshiaki Yamamoto, Norio Iizuka, Katsusuke Naito |
Journal | Anticancer research
(Anticancer Res)
2006 Nov-Dec
Vol. 26
Issue 6B
Pg. 4195-202
ISSN: 0250-7005 [Print] Greece |
PMID | 17201133
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- DNA, Complementary
- Quinazolines
- ErbB Receptors
- Gefitinib
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Topics |
- Antineoplastic Agents
(pharmacology)
- Cell Division
(drug effects)
- Cell Line, Tumor
- DNA, Complementary
- ErbB Receptors
(antagonists & inhibitors)
- Gefitinib
- Gene Expression Profiling
- Humans
- Oligonucleotide Array Sequence Analysis
- Quinazolines
(pharmacology)
- Urinary Bladder Neoplasms
(genetics, pathology)
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