Enhanced resistance to Salmonella enterica serovar Typhimurium infection in mice after coumarin treatment.

Abstract

Coumarin and its derivatives are naturally occurring substances with multiple biological activities. Here we demonstrate that prophylactic peroral administration of coumarin or 7-hydroxycoumarin (7-OHC) enhances resistance to subsequent lethal Salmonella enterica Serovar Typhimurium infection in mice. 7-OHC decreased bacterial load in liver and spleen, and enhanced phagocytosis and bacterial killing by macrophages when applied in vitro and in vivo. 7-OHC treatment induced significant NO release in peritoneal macrophage cultures. The observed protective effect correlated with the induction of Th1-associated cytokines, such as IL-12, IFN-gamma, and TNF-alpha. These data demonstrate a clear immunomodulatory potential of coumarins which might have important therapeutic implications to enhance resistance to infection.

• Authors: Tsvetanka Stefanova, Nadja Nikolova, Anastasija Michailova, Ivan Mitov, Ianco Iancov, Gerhard J Zlabinger, Hristo Neychev
• Journal: Microbes and infection (Microbes Infect) Vol. 9 Issue 1 Pg. 7-14 (Jan 2007) ISSN: 1286-4579 [Print] France
• PMID: 17196418 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Coumarins
• Reactive Nitrogen Species
• Tumor Necrosis Factor-alpha
• Umbelliferones
• Interleukin-12
• 7-hydroxycoumarin
• Interferon-gamma
• coumarin

Topics

• Animals
• Coumarins (blood, immunology, pharmacology)
• Interferon-gamma (immunology)
• Interleukin-12 (immunology)
• Liver (drug effects, immunology, microbiology)
• Macrophages (drug effects, immunology, microbiology)
• Mice
• Mice, Inbred BALB C
• Monocytes (drug effects, immunology)
• Neutrophils (drug effects, immunology)
• Phagocytosis (drug effects, immunology)
• Reactive Nitrogen Species (metabolism)
• Salmonella typhi (immunology, isolation & purification)
• Spleen (drug effects, immunology, microbiology)
• Th1 Cells (drug effects, immunology)
• Tumor Necrosis Factor-alpha (immunology)
• Typhoid Fever (immunology, microbiology, prevention & control)
• Umbelliferones (blood, immunology, pharmacology)