The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells.

Abstract

We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4(+) T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4(+) T cells that secreted elevated levels of IFN-gamma, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to naïve recipients using CPAF-specific CD4(+) T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4(+) T cells.

• Authors: Cathi Murphey, Ashlesh K Murthy, Patricia A Meier, M Neal Guentzel, Guangming Zhong, Bernard P Arulanandam
• Journal: Cellular immunology (Cell Immunol) Vol. 242 Issue 2 Pg. 110-7 (Aug 2006) ISSN: 0008-8749 [Print] Netherlands
• PMID: 17116296 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Bacterial Vaccines
• Interleukin-12
• Endopeptidases
• CPA factor

Topics

• Administration, Intranasal
• Adoptive Transfer
• Animals
• Bacterial Vaccines (administration & dosage, immunology)
• CD4-Positive T-Lymphocytes (cytology, immunology)
• Cell Proliferation
• Chlamydia (enzymology, immunology)
• Endopeptidases (immunology)
• Female
• Interleukin-12 (administration & dosage, immunology)
• Mice
• Mice, Inbred C57BL
• Oviducts (immunology)
• Th1 Cells (immunology)