Reduced Cd4+Cd25+ T cells in patients with idiopathic thrombocytopenic purpura.

Abstract

Immunoregulatory CD4(+)CD25(+) T cells play an important role in the induction and maintenance of peripheral self-tolerance. These professional regulatory cells prevent the activation and proliferation of potentially autoreactive T cells that have escaped thymic deletion. Therefore, CD4(+)CD25(+) T cells are believed to possibly play an important role in pathogenic autoimmune diseases. We measured the count of CD4(+)CD25(+) T cells in 44 patients with idiopathic thrombocytopenic purpura (ITP), and the number of CD4(+)CD25(+) T cells and clinical features were then analyzed. By using a flow cytometric analysis, the number of CD4(+)CD25(+) T cells in the patients with ITP showed a very wide distribution in comparison to healthy volunteers. The number of CD4(+)CD25(+) T cells was significantly lower in the ITP patients in the severe phase, and in patients positive for anti-glycoprotein IIb-IIIa antibody. However, the number of those cells increased in the patients at the complete remission phase, especially after a splenectomy. The Foxp3 mRNA levels of peripheral blood mononuclear cells (PBMC) of ITP patients were higher with an improved platelet count than in those with a low platelet count. In addition, the Foxp3 mRNA levels closely correlated with the number of CD4(+)CD25(+) cells. These mechanisms remain to be fully elucidated, however, the count of CD4(+)CD25(+) T cells is considered to possibly be related to the severity of ITP.

• Authors: Miho Sakakura, Hideo Wada, Isao Tawara, Tsutomu Nobori, Takashi Sugiyama, Norimasa Sagawa, Hiroshi Shiku
• Journal: Thrombosis research (Thromb Res) Vol. 120 Issue 2 Pg. 187-93 ( 2007) ISSN: 0049-3848 [Print] United States
• PMID: 17067661 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• DNA Primers
• FOXP3 protein, human
• Forkhead Transcription Factors
• RNA, Messenger

Topics

• Adult
• Aged
• Aged, 80 and over
• Autoantibodies (blood)
• Base Sequence
• Blood Platelets (immunology)
• Case-Control Studies
• DNA Primers (genetics)
• Female
• Flow Cytometry
• Forkhead Transcription Factors (genetics)
• Humans
• Lymphocyte Count
• Male
• Middle Aged
• Purpura, Thrombocytopenic, Idiopathic (blood, genetics, immunology)
• RNA, Messenger (blood, genetics)
• T-Lymphocytes, Regulatory (immunology)