Ex vivo lentivirus transduction and immediate transplantation of uncultured hepatocytes for treating hyperbilirubinemic Gunn rat.

Abstract

BACKGROUND:

Ex vivo liver gene therapy provides an attractive alternative to orthotopic liver transplantation for the treatment of liver diseases. We previously reported a protocol in which human primary hepatocytes are highly transduced in Suspension with Lentiviral vectors and Immediately Transplanted (SLIT). Here, we evaluated the SLIT approach in Gunn rats, the animal model for Crigler-Najjar syndrome type 1, a defect in bilirubin UDP-glucuronosyltransferase (BUGT).

METHODS:

We constructed lentiviral vectors coding for BUGT under control of an ubiquitous promoter. Control vectors contained Green Fluorescent Protein (GFP) under control of the same promoter. Hepatocytes were isolated from jaundiced Gunn rats and transduced in suspension for four hr. After washing, 2x10 hepatocytes were immediately transplanted into syngeneic rats. Bilirubinemia and bile pigments were regularly assessed after cell transplantation. The percentage and presence of transduced hepatocytes was analyzed by immunohistochemistry in GFP-transplanted animals.

RESULTS:

In rats receiving BUGT-transduced hepatocytes, bilirubinemia decreased by about 30%. The level of correction remained stable for up to 240 days. Bilirubin glucuronides were present in the bile of treated animals, indicating the metabolic activity of engrafted hepatocytes. In contrast, bilirubinemia in GFP-transplanted rats did not decline but rather increased. GFP-positive hepatocytes amounted to 0.5-1% of the liver, which is in agreement with the number of transplanted and genetically-modified hepatocytes (6x10).

CONCLUSIONS:

This work reports the first demonstration of long-term metabolic benefit after rapid transplantation of ex vivo lentivirally tranduced hepatocytes. Therefore, this study demonstrates the therapeutic proof-of-principle and potential of the SLIT approach for treating inherited metabolic liver diseases.

• Authors: Tuan Huy Nguyen, Jacques Birraux, Barbara Wildhaber, Anne Myara, Francois Trivin, Claude Le Coultre, Didier Trono, Christophe Chardot
• Journal: Transplantation (Transplantation) Vol. 82 Issue 6 Pg. 794-803 (Sep 27 2006) ISSN: 0041-1337 [Print] United States
• PMID: 17006327 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• RNA, Viral
• Green Fluorescent Proteins

Topics

• Animals
• Base Sequence
• Cell Line
• Disease Models, Animal
• Genetic Vectors
• Green Fluorescent Proteins (genetics)
• HeLa Cells
• Hepatocytes (transplantation, virology)
• Humans
• Hyperbilirubinemia (surgery)
• Kidney
• Lentivirus (genetics)
• Liver (virology)
• Male
• Promoter Regions, Genetic
• RNA, Messenger (genetics, isolation & purification)
• RNA, Viral (genetics, isolation & purification)
• Rats
• Rats, Gunn
• Transplantation, Isogeneic