Macrophage activation by polysaccharide biological response modifier isolated from Aloe vera L. var. chinensis (Haw.) Berg.

Abstract

A mannose-rich polysaccharide biological response modifier (BRM), derived from Aloe vera L. var. chinensis (Haw.) Berg., was demonstrated to be a potent murine B- and T-cell stimulator in our previous study. We here report the stimulatory activity of PAC-I on murine peritoneal macrophage. The polysaccharide when injected into mice enhanced the migration of macrophages to the peritoneal cavity. Peritoneal macrophage when treated by PAC-I in vitro had increased expression of MHC-II and FcgammaR, and enhanced endocytosis, phagocytosis, nitric oxide production, TNF-alpha secretion and tumor cell cytotoxicity. The administration of PAC-I into allogeneic ICR mice stimulated systemic TNF-alpha production in a dose-dependent manner and prolonged the survival of tumor-bearing mice. PAC-I is thus a potent stimulator of murine macrophage and the in vitro observed tumoricidal properties of activated macrophage might account for the in vivo antitumor properties of PAC-I. Our research findings may have therapeutic implications in tumor immunotherapy.

• Authors: C Liu, M Y K Leung, J C M Koon, L F Zhu, Y Z Hui, B Yu, K P Fung
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 6 Issue 11 Pg. 1634-41 (Nov 2006) ISSN: 1567-5769 [Print] Netherlands
• PMID: 16979117 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Histocompatibility Antigens Class II
• Immunologic Factors
• Polysaccharides
• Receptors, IgG
• Tumor Necrosis Factor-alpha
• Nitric Oxide

Topics

• Aloe (chemistry)
• Animals
• Antineoplastic Agents (pharmacology)
• Cell Line, Tumor
• Histocompatibility Antigens Class II (biosynthesis)
• Immunologic Factors (isolation & purification, pharmacology)
• Macrophage Activation (drug effects)
• Macrophages, Peritoneal (drug effects, immunology, metabolism)
• Mice
• Mice, Inbred BALB C
• Nitric Oxide (biosynthesis)
• Phagocytosis (drug effects)
• Polysaccharides (isolation & purification, pharmacology)
• Receptors, IgG (biosynthesis)
• Tumor Necrosis Factor-alpha (biosynthesis)