[The expression of alpha 1-inhibitor 3 gene and some changes of its structure in rat hepatomas].

Abstract

The expression and structure of alpha 1-inhibitor 3 (alpha 1-I3) gene were investigated in 16 primary rat hepatomas induced by diethylnitrosamine. In most tumor samples (75%, 12/16), the expression of alpha 1-I3 gene manifested markedly diminished or undetectable level of alpha 1-I3 mRNA. Further study indicated that the abnormal expression of alpha 1-I3 gene in the hepatomas examined might be due to, at least in part, the gene hypermethylation, insertion of repeat sequence(s) or base substitutions at the recognition sequences of some restriction endonucleases which caused certain alteration in the gene structure. The significance of alpha 1-I3 as an endogenous antitumor factor in hepatocarcinogenesis was also discussed.

• Authors: Q Z Zhao
• Journal: Shi yan sheng wu xue bao (Shi Yan Sheng Wu Xue Bao) Vol. 24 Issue 4 Pg. 325-31 (Dec 1991) ISSN: 0001-5334 [Print] China
• PMID: 1686700 (Publication Type: English Abstract, Journal Article)

Chemical References

• Acute-Phase Proteins
• DNA Transposable Elements
• Protease Inhibitors
• alpha 1-inhibitor 3
• Diethylnitrosamine
• Phenobarbital

Topics

• Acute-Phase Proteins
• Animals
• DNA Transposable Elements
• Diethylnitrosamine
• Female
• Gene Expression Regulation, Neoplastic
• Liver Neoplasms, Experimental (chemically induced, genetics)
• Male
• Methylation
• Phenobarbital
• Polymorphism, Restriction Fragment Length
• Protease Inhibitors (metabolism)
• Rats
• Rats, Inbred Strains