[Effect of anti-Helicobacter pylori ureB monoclonal antibody on platelet aggregation and activation, and its mechanism study].

Abstract

OBJECTIVES:

To study the effect of monoclonal antibody (McAb) against helicobacter pylori (Hp) ureB, 1F11 on platelet aggregation and activation, and its mechanism.

METHODS:

The relativity between human platelet glycoproteins (GPs) and Hp ureB was identified by Western blot and FCM. Platelet aggregation was measured by turbidimetry, and P-selectin and TXB2 assay by ELISA.

RESULTS:

1F11 could bind to platelet GPIIIa, and ADP-induced platelet aggregation was inhibited by 1F11 in a dose-dependent manner. However, 1F11 had no effect on plasma P-selectin and TXB2 induced by ADP. The FCM results show that the positive rates of platelet binding to FITC-SZ21 was decreased from 99.5% to 77.4% after addition of 1F11.

CONCLUSION:

McAb against Hp ureB 1F11 inhibits platelet aggregation through binding to platelet GPIIIa but does not block platelet activation. There might be crossed-epitopes on Hp ureB and platelet GPIIIa, and Hp infection might be involved in ITP immunopathology.

• Authors: Yan-yan Bai, Zhao-yue Wang, Xia Bai, Jing-cheng Miao, Wei Zhang, Lan Dai, Wen-hong Shen, Chang-geng Ruan
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 27 Issue 3 Pg. 166-9 (Mar 2006) ISSN: 0253-2727 [Print] China
• PMID: 16792917 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antibodies, Monoclonal
• Bacterial Proteins
• Integrin beta3
• P-Selectin
• Urokinase-Type Plasminogen Activator
• Urease

Topics

• Antibodies, Bacterial (pharmacology)
• Antibodies, Monoclonal (pharmacology)
• Bacterial Proteins (immunology, metabolism)
• Helicobacter pylori (immunology)
• Humans
• Integrin beta3 (immunology)
• P-Selectin (immunology)
• Platelet Activation (drug effects)
• Platelet Aggregation (drug effects)
• Urease (immunology)
• Urokinase-Type Plasminogen Activator (immunology)