Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana.

Abstract

BiP is a molecular chaperone induced in the unfolded protein response (UPR). In mammalian cells, BiP is induced by glucose starvation when it is called glucose-regulated protein 78 (GRP78). In Arabidopsis thaliana, however, we demonstrated that BiP transcripts decreased with sugar depletion and increased with sugar addition. Transcripts for beta-glucuronidase (GUS) driven by BiP promoter respond to tunicamycin and sugar, being similar with endogenous BiP transcripts in transgenic A. thaliana. When GUS was regulated by P-UPRE, a cis-element responsible for the UPR identified in BiP promoter, GUS transcripts were accumulated by sugar starvation. Subsequently, transgenic A. thaliana harboring luciferase (LUC) gene regulated by P-UPRE was analyzed. Sugar depletion also increased LUC activity. It is concluded that BiP is induced by sugar independent of the cis-element responsible for the UPR.

• Authors: Hiromi Tajima, Nozomu Koizumi
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 346 Issue 3 Pg. 926-30 (Aug 04 2006) ISSN: 0006-291X [Print] United States
• PMID: 16781668 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Arabidopsis Proteins
• Carbohydrates
• Carrier Proteins
• Recombinant Proteins
• Tunicamycin
• BIP protein, Arabidopsis
• Glucuronidase

Topics

• Arabidopsis (drug effects, genetics, metabolism)
• Arabidopsis Proteins (biosynthesis, genetics)
• Carbohydrates (pharmacology)
• Carrier Proteins (biosynthesis, genetics)
• Gene Expression Regulation, Plant
• Genes, Reporter (genetics)
• Glucuronidase (genetics, metabolism)
• Plants, Genetically Modified
• Promoter Regions, Genetic (genetics)
• Protein Folding
• Recombinant Proteins (biosynthesis, genetics)
• Transcription, Genetic (genetics)
• Tunicamycin (pharmacology)