Dexamethasone decreases xenograft response to Paclitaxel through inhibition of tumor cell apoptosis.

Abstract

Glucocorticoid receptor (GR) activation has recently been implicated in the initiation of anti-apoptotic signaling pathways in epithelial cell lines grown in culture. However, the evidence that GR-mediated inhibition of tumor cell apoptosis is the mechanism that diminishes chemotherapy effectiveness in vivo is limited. We therefore initiated a breast cancer xenograft study to examine whether or not pretreatment with glucocorticoids (GCs) decreases tumor response to chemotherapy by inhibiting tumor cell apoptosis. Here we report a significant decrease in paclitaxel-induced apoptosis in xenografts from mice pretreated with dexamethasone (Dex). A significant difference in apoptosis in xenografts from Dex/paclitaxel versus paclitaxel treated animals was seen eight days following initiation of chemotherapy. Nine days later, mice treated with Dex/paclitaxel had significantly larger tumors compared with those that received paclitaxel alone (p = 0.032). Dex pretreatment did not significantly affect tumor cell proliferation rates. Taken together, these results demonstrate that systemic Dex administration results in significantly reduced breast cancer xenograft apoptosis in the context of chemotherapy treatment. We also found that systemic Dex treatment results in upregulation of the anti-apoptotic gene MKP-1 and downregulation of pro-apoptotic Bid and TRAIL genes in tumor cells six hours following Dex treatment. These in vivo gene expression changes correlated with significant inhibition of chemotherapy-induced apoptosis. Interestingly, the decreased chemotherapeutic response of Dex-pretreated tumors persisted for several weeks following treatment. These data suggest that GR-mediated transcriptional regulation of pro- and anti-apoptotic genes contributes to the mechanism through which GCs decrease paclitaxel-induced apoptosis.

• Authors: Diana Pang, Masha Kocherginsky, Thomas Krausz, So-Young Kim, Suzanne D Conzen
• Journal: Cancer biology & therapy (Cancer Biol Ther) Vol. 5 Issue 8 Pg. 933-40 (Aug 2006) ISSN: 1538-4047 [Print] United States
• PMID: 16775428 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Antineoplastic Agents, Phytogenic
• BH3 Interacting Domain Death Agonist Protein
• Bid protein, mouse
• Glucocorticoids
• Immediate-Early Proteins
• RNA, Messenger
• Receptors, Glucocorticoid
• TNF-Related Apoptosis-Inducing Ligand
• Dexamethasone
• Protein Serine-Threonine Kinases
• serum-glucocorticoid regulated kinase
• Mitogen-Activated Protein Kinases
• Phosphoprotein Phosphatases
• Paclitaxel

Topics

• Animals
• Antineoplastic Agents, Phytogenic (therapeutic use)
• Apoptosis (drug effects)
• BH3 Interacting Domain Death Agonist Protein (genetics, metabolism)
• Dexamethasone (therapeutic use)
• Enzyme Activation (drug effects)
• Female
• Gene Expression Regulation, Neoplastic
• Glucocorticoids (therapeutic use)
• Humans
• Immediate-Early Proteins (metabolism)
• Immunoenzyme Techniques
• In Situ Nick-End Labeling
• Mammary Neoplasms, Experimental (drug therapy, metabolism, pathology)
• Mice
• Mice, SCID
• Mitogen-Activated Protein Kinases (genetics, metabolism)
• Paclitaxel (therapeutic use)
• Phosphoprotein Phosphatases (genetics, metabolism)
• Protein Serine-Threonine Kinases (metabolism)
• RNA, Messenger (metabolism)
• Receptors, Glucocorticoid (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• TNF-Related Apoptosis-Inducing Ligand (genetics, metabolism)
• Transplantation, Heterologous
• Tumor Cells, Cultured