Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein.

Abstract

Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-gamma in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.

• Authors: Lisa F Shubitz, Jieh-Juen Yu, Chiung-Yu Hung, Theo N Kirkland, Tao Peng, Robert Perrill, Julie Simons, Jianmin Xue, Roger A Herr, Garry T Cole, John N Galgiani
• Journal: Vaccine (Vaccine) Vol. 24 Issue 31-32 Pg. 5904-11 (Jul 26 2006) ISSN: 0264-410X [Print] Netherlands
• PMID: 16759762 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Fungal Proteins
• Fungal Vaccines
• Vaccines, Subunit
• Vaccines, Synthetic

Topics

• Amino Acid Sequence
• Animals
• Coccidioides
• Coccidioidomycosis (microbiology, prevention & control)
• Female
• Fungal Proteins (genetics, therapeutic use)
• Fungal Vaccines (therapeutic use)
• Mice
• Mice, Inbred C57BL
• Molecular Sequence Data
• Respiratory Tract Infections (microbiology, prevention & control)
• Vaccines, Subunit (therapeutic use)
• Vaccines, Synthetic (therapeutic use)