The effects of alpha-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimurium and Shigella flexneri.

Abstract

Two milk components, alpha-lactalbumin (alpha-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. (3)[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested alpha-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with alpha-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.

• Authors: Wolfram M Brück, Shannon L Kelleher, Glenn R Gibson, Gitte Graverholt, Bo L Lönnerdal
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 259 Issue 1 Pg. 158-62 (Jun 2006) ISSN: 0378-1097 [Print] England
• PMID: 16684117 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycopeptides
• Milk Proteins
• Lactalbumin

Topics

• Bacterial Adhesion (drug effects)
• Caco-2 Cells (microbiology)
• Escherichia coli (drug effects, pathogenicity)
• Glycopeptides (pharmacology)
• Humans
• Lactalbumin (pharmacology)
• Milk Proteins (chemistry, pharmacology)
• Salmonella typhimurium (drug effects, pathogenicity)
• Shigella flexneri (drug effects, pathogenicity)