Selective inhibition of endoplasmic reticulum-associated degradation rescues DeltaF508-cystic fibrosis transmembrane regulator and suppresses interleukin-8 levels: therapeutic implications.

Abstract

Endoplasmic reticulum (ER)-associated degradation (ERAD) is the major quality control pathway of the cell. The most common disease-causing protein folding mutation, DeltaF508-cystic fibrosis transmembrane regulator (CFTR), is destroyed by ERAD to cause cystic fibrosis (CF). p97/valosin-containing protein (VCP) physically interacts with gp78/autocrine motility factor receptor to couple ubiquitination, retrotranslocation, and proteasome degradation of misfolded proteins. We show here that p97/VCP and gp78 form complexes with CFTR during translocation from the ER for degradation by the cytosolic proteasome. Interference in the VCP-CFTR complex promoted accumulation of immature CFTR in the ER and partial rescue of functional chloride channels to the cell surface. Moreover, under these conditions, interleukin-8 (IL8), the expression of which is regulated by the proteasome, was reduced. Inhibition of the proteasome with bortezomib (PS-341/Velcade) also rescued CFTR, but with less efficiency, and suppressed NFkappaB-mediated IL8 activation. The inhibition of the major stress-inducible transcription factor CHOP (CCAAT/enhancer-binding protein homologous protein)/GADD153 together with bortezomib was most effective in repressing NFkappaB-mediated IL8 activation compared with interference of VCP, MLN-273 (proteasome inhibitor), or 4-phenylbutyrate (histone deacetylase inhibitor). Immunoprecipitation of DeltaF508-CFTR from primary CF bronchial epithelial cells confirmed the interaction with VCP and associated chaperones in CF. We conclude that VCP is an integral component of ERAD and cellular stress pathways induced by the unfolded protein response and may be central to the efficacy of CF drugs that target the ubiquitin-proteasome pathway.

• Authors: Neeraj Vij, Shengyun Fang, Pamela L Zeitlin
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 281 Issue 25 Pg. 17369-17378 (Jun 23 2006) ISSN: 0021-9258 [Print] United States
• PMID: 16621797 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Cell Cycle Proteins
• Enzyme Inhibitors
• Interleukin-8
• NF-kappa B
• Proteasome Inhibitors
• Cystic Fibrosis Transmembrane Conductance Regulator
• Proteasome Endopeptidase Complex
• Adenosine Triphosphatases
• VCP protein, human
• Valosin Containing Protein

Topics

• Adenosine Triphosphatases
• Adolescent
• Cell Cycle Proteins (metabolism)
• Child
• Child, Preschool
• Cystic Fibrosis (metabolism, therapy)
• Cystic Fibrosis Transmembrane Conductance Regulator (genetics, metabolism)
• Endoplasmic Reticulum (metabolism)
• Enzyme Inhibitors (pharmacology)
• Humans
• Interleukin-8 (biosynthesis, metabolism)
• NF-kappa B (metabolism)
• Proteasome Endopeptidase Complex (metabolism)
• Proteasome Inhibitors
• Valosin Containing Protein