[Inhibitory effect of p53 gene on telomerase activity and proliferative activity in salivary adenoid cystic carcinoma cells].

Abstract

OBJECTIVE:

To evaluate the inhibitory effect of p53 gene on salivary adenoid cystic carcinoma (SACC) cells.

METHODS:

Adenoviral vector pDeltaE1-p53 was constructed and transfected into SACC-83 cells. The enhanced p53 expression was measured by reverse transcription polymerase chain reaction (RT-PCR), and the effects of transfected p53 on SACC-83 cells were analyzed by TRAP-PCR-ELISA, luciferase reporter, flow cytometry (FCM), soft agar assay and tumorigenicity test.

RESULTS:

The expression of p53 gene in SACC-83 cells was increased after introduction of pDeltaE1-p53. The telomerase activity and the transcriptional activity of hTERT promoter were inhibited. The cells cycles of transfected SACC-83 were arrested in G(1) phase and the rate of colony-formation was decreased, and similarly the tumorigenicity in nude mice was also decreased.

CONCLUSIONS:

The introduction of wild-type p53 by adenoviral vector could suppress the telomerase activity and malignant phenotypes of salivary adenoid cystic carcinoma cells.

• Authors: Bing-zhi Yan, Jie Wang, Bo Zhang, Fu-sheng Dong, Lin Hou, Xu Wang
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 41 Issue 1 Pg. 45-8 (Jan 2006) ISSN: 1002-0098 [Print] China
• PMID: 16620628 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tumor Suppressor Protein p53
• Telomerase

Topics

• Animals
• Carcinoma, Adenoid Cystic (genetics, metabolism, pathology)
• Cell Line, Tumor
• Genetic Vectors
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Salivary Gland Neoplasms (genetics, metabolism, pathology)
• Telomerase (genetics, metabolism)
• Transfection
• Tumor Suppressor Protein p53 (genetics)