[In vitro killing effects of STI571 on esophageal carcinoma cell lines CE-48T and CE-81T].

Abstract

BACKGROUND & OBJECTIVE:

Tyrosine kinase mediates cell proliferation and differentiation, and plays important roles in tumorigenesis and development of esophageal carcinoma. STI571 is a tyrosine kinase inhibitor of platelet-derived growth factor receptor beta (PDGFR-beta) which is overexpressed in esophageal carcinoma. This study was to explore the in vitro killing effects of STI571 on esophageal carcinoma cell lines CE-48T and CE-81T.

METHODS:

The expression of PDGFR-alpha and PDGFR-beta in CE-48T and CE-81T cells was detected by Western blot. The killing effects of STI571 on CE-48T and CE-81T cells were evaluated by MTT assay. Cell apoptosis was analyzed by flow cytometry with Annexin V/PI labeling. The expression of p-PDGFR-beta was detected by Western blot before and after treatment of STI571.

RESULTS:

CE-48T cells expressed PDGFR-beta, but did not express PDGFR-alpha; CE-81T cells did not express both PDGFR-alpha and PDGFR-beta. The 50% inhibitory concentration (IC50) of STI571 was significantly lower for CE-48T cells than for CE-81T cells [(8.32+/-1.50) micromol/L vs. (41.02+/-7.64) micromol/L, P=0.002]. When treated with 10 micromol/L STI571 for 12 h, the apoptosis rate of CE-48T cells was (52.43+/-5.30)%, but the apoptosis rate did not increase as the treatment time and concentration increased. After treatment of STI571, the expression of p-PDGFR-beta was inhibited in CE-48T cells, but didn't change in CE-81T cells.

CONCLUSIONS:

STI-571 could induce the apoptosis of PDGFR-beta-positive esophageal carcinoma CE-48T cells. p-PDGFR-beta might be the target of STI571.

• Authors: Peng-Yuan Zhang, Bao-Jiang Li, Xiao-Dong Su, Zhe-Sheng Wen, Jin-Ming Zhao, Lan-Jun Zhang, Hao Long, Tie-Hua Rong
• Journal: Ai zheng = Aizheng = Chinese journal of cancer (Ai Zheng) Vol. 25 Issue 4 Pg. 456-60 (Apr 2006) China
• PMID: 16613680 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Benzamides
• Piperazines
• Pyrimidines
• Imatinib Mesylate
• Protein-Tyrosine Kinases
• Receptor, Platelet-Derived Growth Factor alpha
• Receptor, Platelet-Derived Growth Factor beta

Topics

• Antineoplastic Agents (administration & dosage, pharmacology)
• Apoptosis (drug effects)
• Benzamides
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Drug Delivery Systems
• Esophageal Neoplasms (pathology)
• Humans
• Imatinib Mesylate
• Piperazines (administration & dosage, pharmacology)
• Protein-Tyrosine Kinases (antagonists & inhibitors)
• Pyrimidines (administration & dosage, pharmacology)
• Receptor, Platelet-Derived Growth Factor alpha (metabolism)
• Receptor, Platelet-Derived Growth Factor beta (metabolism)