Crystallization and preliminary X-ray crystallographic analysis of osteoclast-stimulating factor.

Abstract

Osteoclast-stimulating factor increases osteoclast formation and bone resorption through a cellular signal transduction cascade, possibly by its interaction with c-Src or related family members. Crystals of human osteoclast-stimulating factor were obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant. The crystals are primitive orthorhombic and belong to P222 or a related space group, with unit-cell parameters a = 38.1, b = 54.9, c = 64.7 A.

• Authors: Ming Li, Zhaohui Meng, Yanhui Xu, Zhiyong Lou, Zihe Rao
• Journal: Acta crystallographica. Section F, Structural biology and crystallization communications (Acta Crystallogr Sect F Struct Biol Cryst Commun) Vol. 61 Issue Pt 1 Pg. 128-30 (Jan 01 2005) ISSN: 1744-3091 [Electronic] England
• PMID: 16508112 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Intracellular Signaling Peptides and Proteins
• Peptides
• osteoclast stimulating factor

Topics

• Base Sequence
• Chromosomes, Human, Pair 12
• Crystallization
• Crystallography, X-Ray
• DNA Primers
• Humans
• Intracellular Signaling Peptides and Proteins
• Peptides (chemistry, genetics, isolation & purification)
• X-Ray Diffraction