Abstract |
Three antimicrobial peptides exhibiting in vitro antifungal activity were expressed in Arabidopsis to compare their in planta activity. Beta-Purothionin, cecropin B, and phor21 were expressed under an endogenous promoter with moderate-level activity and excreted extracellularly. Expression of beta-purothionin rendered the greatest antibacterial and antifungal resistance while cecropin B enhanced only antibacterial activity and phor21 did not improve antimicrobial resistance. The transgenic beta-purothionin arrested fungal growth on leaf surfaces and infection of stomata. Leaf extracts from plants producing beta-purothionin and cecropin B displayed membrane permeabilizing activity. The in planta antimicrobial activity of the tested peptides was consistent with previously reported in vitro experiments. The expression strategy allowed enhanced antifungal resistance without high-level transgene expression.
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Authors | S V Oard, F M Enright |
Journal | Plant cell reports
(Plant Cell Rep)
Vol. 25
Issue 6
Pg. 561-72
(Jun 2006)
ISSN: 0721-7714 [Print] Germany |
PMID | 16456649
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimicrobial Cationic Peptides
- Insect Proteins
- Plant Proteins
- cecropin B protein, Insecta
- purothionin
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Topics |
- Antimicrobial Cationic Peptides
(biosynthesis, genetics)
- Arabidopsis
(genetics, metabolism, microbiology)
- Fusarium
- Insect Proteins
(biosynthesis, genetics)
- Plant Leaves
(genetics, metabolism, microbiology)
- Plant Proteins
(biosynthesis, genetics)
- Plants, Genetically Modified
- Promoter Regions, Genetic
- Pseudomonas syringae
- Rhizoctonia
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