A novel apoptosis-inducing monoclonal antibody (anti-LHK) against a cell surface antigen on colon cancer cells.

Abstract

BACKGROUND:

Apoptosis is a crucial element in the behavior of mammalian cells in many different situations. We here report the establishment of a novel monoclonal antibody (anti-LHK mAb) that has apoptosis-inducing activity against colon cancer Colo205 cells.

METHODS:

The mechanism of anti-LHK mAb-induced cell death was assessed by microscopic morphology, Annexin V/Hoechst 33528 staining, and detection of DNA fragmentation. The molecular weight of LHK antigen was determined by Western blotting. Growth inhibition of Colo205 cells induced by anti-LHK mAb was determined by in vitro and in vivo studies.

RESULTS:

Anti-LHK reacted with a 70-kDa antigen and completely blocked the proliferation of Colo205 cells bearing LHK in vitro in a manner characteristic of apoptosis. Strikingly, anti-LHK mAb suppressed tumor growth in a murine peritoneal dissemination model.

CONCLUSIONS:

LHK antigen, which is restricted to epithelial cells, may be a novel death receptor that plays a critical role in controlling the growth, invasion, and metastasis of human colon cancer cells.

• Authors: Hidehiko Matsukawa, Takanori Kanai, Makoto Naganuma, Nobuhiko Kamada, Tadakazu Hisamatsu, Hiromasa Takaishi, Haruhiko Ogata, Makio Mukai, Hiromasa Ishii, Tsuneyo Mimori, Mamoru Watanabe, Toshifumi Hibi
• Journal: Journal of gastroenterology (J Gastroenterol) Vol. 40 Issue 10 Pg. 945-55 (Oct 2005) ISSN: 0944-1174 [Print] Japan
• PMID: 16261431 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Surface

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antigens, Surface
• Apoptosis (immunology)
• Cells, Cultured
• Colonic Neoplasms (immunology, pathology)
• Humans
• Mice
• Mice, Inbred BALB C