Pilot study to assess the presence of Chlamydia trachomatis in urine from 18-30-year-old males using EIA/IF and PCR.

Abstract

CONTEXT:

To increase detection, urine samples from young males could be opportunistically tested for Chlamydia trachomatis.

OBJECTIVE:

To determine C. trachomatis prevalence in urine, optimum specimen and compare sensitivity/feasibility of routine use of different testing methods.

DESIGN:

Group A, 'sterile' pyuria samples June 1998-January 1999, tested by enzyme immunoassay (EIA) and, if reactive, by immunofluorescence (IF). Subsequently batch-tested by polymerase chain reaction (PCR). Group B, consecutive urine samples October 1998-January 1999; batch-tested by PCR.

SETTING:

Microbiology laboratory.

SAMPLES:

From males aged 18-30 years; group A = 71, group B = 83.

MAIN OUTCOME MEASURES:

Chlamydia trachomatis positive if EIA- and IF- or PCR-positive.

RESULTS:

Group A: 12 EIA/IF-positive; 9/12 and 15 EIA-negative samples PCR-positive. Group B: 11 PCR-positive; 8/11 showed 'sterile' pyuria.

CONCLUSIONS:

Opportunistic testing of urine from young men shows a significant number of C. trachomatis infections. 'Sterile' pyuria samples are optimal. EIA/IF are less sensitive than PCR but can be routinely performed and detect a significant proportion of cases.

• Authors: Adriana Basarab, David Browning, Stuart Lanham, Susan O'Connell
• Journal: The journal of family planning and reproductive health care (J Fam Plann Reprod Health Care) Vol. 28 Issue 1 Pg. 36-7 (Jan 2002) ISSN: 1471-1893 [Print] England
• PMID: 16259815 (Publication Type: Journal Article)

Topics

• Adolescent
• Adult
• Chlamydia Infections (epidemiology, urine)
• Chlamydia trachomatis (isolation & purification)
• Humans
• Immunoenzyme Techniques
• Male
• Pilot Projects
• Polymerase Chain Reaction
• Prevalence
• Sensitivity and Specificity
• United Kingdom (epidemiology)