Abstract | PURPOSE: EXPERIMENTAL DESIGN: Fifty NAF samples, 25 from breasts with cancer and 25 from normal breasts, were examined. Antigen capture immunoassays were done on the samples using monoclonal antibodies that specifically recognized either TF or Tn antigen in NAF. These antibodies captured serially diluted NAF samples, and the concentration of TF or Tn was determined by comparing absorbance values against a standard curve generated from standard sources of TF or Tn. RESULTS: TF and Tn were detected in 19 of 25 and 20 of 25 NAF samples from breasts with cancer, respectively, compared with 0 of 25 and 1 of 25 NAF samples from breasts without cancer (P < 0.001 for both TF and Tn). In 92% of the cancerous breast NAF samples tested, either TF or Tn was found. CONCLUSIONS: Simultaneous measurement of TF and Tn in NAF may facilitate the noninvasive detection of breast cancer and warrants further study.
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Authors | Senthil R Kumar, Edward R Sauter, Thomas P Quinn, Susan L Deutscher |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 11
Issue 19 Pt 1
Pg. 6868-71
(Oct 01 2005)
ISSN: 1078-0432 [Print] United States |
PMID | 16203776
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antigens, Neoplasm
- Antigens, Tumor-Associated, Carbohydrate
- Biomarkers, Tumor
- Carbohydrates
- Tn antigen
- Thomsen-Friedenreich antigen
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Topics |
- Adult
- Aged
- Antigens, Neoplasm
(metabolism)
- Antigens, Tumor-Associated, Carbohydrate
(biosynthesis)
- Biomarkers, Tumor
- Biopsy, Needle
- Body Fluids
(metabolism)
- Breast
(metabolism)
- Breast Neoplasms
(diagnosis, metabolism)
- Carbohydrates
(chemistry)
- Enzyme-Linked Immunosorbent Assay
- Female
- Glycosylation
- Humans
- Immunoassay
- Mass Screening
- Middle Aged
- Postmenopause
- Premenopause
- Recurrence
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