Abstract |
The aim of this study was to investigate the efficacy of naringin and naringenin on endotoxin- induced uveitis (EIU) in rats. EIU was induced in male Lewis rats by a footpad injection of lipopolysaccharide (LPS). The rats were injected intravenously with 0.4, 4, or 40 microg/kg naringin or naringenin. Each compound was administered three times, simultaneously, 30 min before and after the actual LPS injection. The aqueous humor was collected 24 h later from both eyes, and the number of cells infiltrating into the aqueous humor and the aqueous humor protein concentration were measured. The levels of prostaglandin E2 ( PGE2) and nitric oxide (NO) were determined. Naringin and naringenin suppressed the development of EIU in a dose-dependent fashion. Both treatments with naringin and naringenin produced reductions in PGE2 and NO concentrations in the aqueous humor. In particular, 40 microM/kg of naringin and naringenin suppressed increases in cell count owing to LPS treatment by 31% and 38%, respectively. The possible mechanism for the antiocular inflammatory effect may be the suppression of PGE2 and NO by naringin and naringenin.
|
Authors | Kenji Shiratori, Kazuhiro Ohgami, Iliyana Ilieva, Xue-Hai Jin, Kazuhiko Yoshida, Satoru Kase, Shigeaki Ohno |
Journal | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
(J Ocul Pharmacol Ther)
Vol. 21
Issue 4
Pg. 298-304
(Aug 2005)
ISSN: 1080-7683 [Print] United States |
PMID | 16117693
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Flavanones
- Lipopolysaccharides
- Nitric Oxide
- naringenin
- Dinoprostone
- naringin
|
Topics |
- Animals
- Aqueous Humor
(cytology, drug effects, metabolism)
- Dinoprostone
(metabolism)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Drug Administration Schedule
- Drug Therapy, Combination
- Flavanones
(administration & dosage, therapeutic use)
- Lipopolysaccharides
(isolation & purification, toxicity)
- Male
- Nitric Oxide
(metabolism)
- Rats
- Rats, Inbred Lew
- Salmonella typhimurium
(metabolism)
- Uveitis, Anterior
(chemically induced, pathology, prevention & control)
|