Abstract |
Rutin, one of the flavonoids derived from plants, is increasingly in demand in the food, pharmaceutical, and cosmetic industries due to its various biological and physiological activities including antioxidation, anti- inflammation, and anti- hypertension. The whole plant of buckwheat (Fagopyrum esculentum Moench) is a major source of natural rutin. This study developed a low-cost process encompassing the efficient extraction, fractionation, and recrystallization to obtain high-purity rutin from buckwheat, and it could improve the economic utilization of this abundant low-value agricultural product. The sequential separation and purification procedures established in this study involved extraction with 50% (v/v) aqueous ethanol at 80 degrees C for 1 h followed by elution with water and aqueous ethanols at 20% and 30% (v/v) on a styrene-based resin column, and recrystallization at 4 degrees C for 12 h. These conditions resulted in the recovery of 92% of total rutin with over 95% purity. In the present study, high-purity rutin was obtained from whole buckwheat through low-cost processes, the separation and purification strategy established in this study could provide valuable information to the relevant industries.
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Authors | Kyoung Heon Kim, Ki Won Lee, Dong Young Kim, Hyung Hwan Park, Ik Boo Kwon, Hyong Joo Lee |
Journal | Bioresource technology
(Bioresour Technol)
Vol. 96
Issue 15
Pg. 1709-12
(Oct 2005)
ISSN: 0960-8524 [Print] England |
PMID | 16023574
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Extracts
- Ethanol
- Rutin
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Topics |
- Chemical Fractionation
- Chromatography, High Pressure Liquid
- Crystallization
- Ethanol
- Fagopyrum
(chemistry)
- Plant Extracts
(isolation & purification)
- Rutin
(analysis, chemistry, isolation & purification)
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