[Analysis of sequence-tagged site in bcr and abl genes by DNA pooling and dHPLC].

Abstract

To investigate the relationship between the single nucleotide polymorphism (SNPs) of the bcr and abl gene and chronic myelogeous leukemia (CML), the 9 sequence-tagged sites (STS) in bcr and abl gene were screened by DNA pooling and denaturing high performance liquid chromatography (dHPLC), and the results were varified by sequencing. The results showed that the polymorphism sites were detected in 4 out of the 9 STS fragments and there were 3 bases different from the reference sequence found in 3 fragments. In conclusion, the novel SNP in U07000 fragment shows significantly different frequencies between CML and controled people.

• Authors: Hong Tian, Dao-Ming Liu, Bing Xu, Wei-Yang Zheng, Shu-Yun Zhou
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 13 Issue 3 Pg. 468-71 (Jun 2005) ISSN: 1009-2137 [Print] China
• PMID: 15972144 (Publication Type: English Abstract, Journal Article)

Chemical References

• Fusion Proteins, bcr-abl
• Proto-Oncogene Proteins c-bcr

Topics

• Chromatography, High Pressure Liquid (methods)
• Fusion Proteins, bcr-abl (genetics)
• Genes, abl (genetics)
• Humans
• Leukemia, Myelogenous, Chronic, BCR-ABL Positive (genetics)
• Polymorphism, Single Nucleotide
• Proto-Oncogene Proteins c-bcr (genetics)
• Sequence Analysis, DNA
• Sequence Tagged Sites