Urinary levels of soluble e-cadherin in the detection of transitional cell carcinoma of the urinary bladder.

Abstract

OBJECTIVE:

To test the hypothesis that elevated urinary levels of soluble E-cadherin (sE-cadherin) would aid in the detection of transitional cell carcinoma (TCC) of the urinary bladder.

METHODS:

We performed sE-cadherin staining of one murine (MBT2) and four human (RT4, 5637, T24, and TCCSUP) bladder cancer cell lines. sE-cadherin levels were also determined in voided urine of 188 consecutive subjects at risk for TCC recurrence, 31 patients with other uro-pathologic conditions, and 10 healthy subjects using a commercially-available ELISA kit. sE-cadherin was analyzed continuously and categorically on the basis of its median distribution.

RESULTS:

Moderately and poorly differentiated bladder cancer cell lines had decreased cellular E-cadherin expression, whereas RT4, a well differentiated cell line, had preserved expression. All cell lines had measurable sE-cadherin levels in their conditioned media. The area under the ROC curve of sE-cadherin for the detection of TCC was 0.719 (95%CI, 0.637-0.801; p<0.001). Higher levels of sE-cadherin were associated with positive cytology results (p=0.012) and muscle invasive tumor stage (p=0.009). Urinary sE-cadherin was more sensitive, but less specific than urinary cytology for the detection of bladder TCC. In a multivariable logistic regression analysis, higher sE-cadherin and positive cytology were both associated with an increased risk of bladder TCC (p=0.048 and p<0.001, respectively). Combination of cytology and sE-cadherin allowed categorization of patients into three significantly different risk groups for bladder cancer. Adjustment of sE-cadherin for urinary creatinine levels did not affect any of the outcomes.

CONCLUSIONS:

Urinary level of sE-cadherin may add information to cytology in the detection of bladder TCC.

• Authors: Shahrokh F Shariat, Kazumasa Matsumoto, Roberto Casella, Weiguo Jian, Seth P Lerner
• Journal: European urology (Eur Urol) Vol. 48 Issue 1 Pg. 69-76 (Jul 2005) ISSN: 0302-2838 [Print] Switzerland
• PMID: 15967254 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Biomarkers, Tumor
• Cadherins
• Culture Media, Conditioned
• Creatinine

Topics

• Adult
• Aged
• Aged, 80 and over
• Animals
• Antibodies, Monoclonal
• Biomarkers, Tumor (biosynthesis, urine)
• Cadherins (biosynthesis, urine)
• Carcinoma, Transitional Cell (pathology, urine)
• Cell Line, Tumor (metabolism)
• Creatinine (urine)
• Culture Media, Conditioned (chemistry)
• Cystoscopy
• Female
• Humans
• Male
• Mice
• Middle Aged
• Predictive Value of Tests
• Risk Factors
• Urinary Bladder Neoplasms (pathology, urine)