Robust hepatitis C virus infection in vitro.

Abstract

The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.

• Authors: Jin Zhong, Pablo Gastaminza, Guofeng Cheng, Sharookh Kapadia, Takanobu Kato, Dennis R Burton, Stefan F Wieland, Susan L Uprichard, Takaji Wakita, Francis V Chisari
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 102 Issue 26 Pg. 9294-9 (Jun 28 2005) ISSN: 0027-8424 [Print] United States
• PMID: 15939869 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, CD
• CD81 protein, human
• DNA, Complementary
• Tetraspanin 28
• Sucrose
• RNA

Topics

• Antigens, CD (biosynthesis)
• Cell Culture Techniques (methods)
• Cell Line
• Cells, Cultured
• Centrifugation, Density Gradient
• Cloning, Molecular
• DNA, Complementary (metabolism)
• Fluorescent Antibody Technique, Indirect
• HL-60 Cells
• HeLa Cells
• Hepacivirus (metabolism)
• Hepatitis C (pathology)
• Humans
• Kinetics
• Microscopy, Fluorescence
• Protein Binding
• RNA (chemistry)
• Sucrose (pharmacology)
• Tetraspanin 28
• Time Factors
• Transcription, Genetic
• Transfection
• U937 Cells